Persistent bacterial colonization of Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans in periodontitis and its association with alveolar bone loss after 6 months of therapy
Persistent bacterial colonization of Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans in periodontitis and its association with alveolar bone loss after 6 months of therapy
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DOI：
10.1034/j.1600-051x.2000.027012897.x
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发表时间：
2000-12-01
影响因子：
6.7
通讯作者：
Snyder, B
中科院分区：
文献类型：
--
作者：
Chaves, ES;Jeffcoat, MK;Snyder, B
背景、目的：本研究旨在确定牙周炎患者在牙周治疗后，其龈下菌斑中牙龈卟啉单胞菌（Pg）、中间普氏菌（Pi）和伴放线放线杆菌（Aa）的细菌抗原存在是否与进行性牙槽骨丧失相关。
方法：对39名总体健康状况良好的受试者进行了研究，这些受试者在过去2年内曾被诊断为成人牙周炎，且仍有2 - 6颗牙齿的探诊深度>5mm。所有受试者均接受了洁治和根面平整治疗。其中一半受试者被随机分配接受辅助性全身性多西环素治疗（第1天200mg，之后21天每天100mg）。在基线、治疗后1个月、3个月和6个月采集龈下菌斑样本。采用改良的酶联免疫吸附测定试验（Evalusite™，牙周检测试剂盒，伊士曼柯达公司，纽约罗切斯特）检测与牙龈卟啉单胞菌、中间普氏菌和伴放线放线杆菌相关的菌斑抗原。使用数字减影放射成像技术，通过在基线和治疗后6个月拍摄的标准化X线片来确定进行性牙槽骨丧失情况。
结果：治疗后菌斑中牙龈卟啉单胞菌的存在与进行性骨丧失显著相关（阳性预测值84%，阴性预测值85%，比值比31.9，p
Background, aims: The purpose of this study was to determine whether the presence of bacterial antigens for Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), and Actinobacillus actinomycetemcomitans (Aa) in subgingival plaque of periodontitis patients after periodontal treatment was associated with progressive alveolar bone loss.Method: 39 (39) subjects in good general health previously diagnosed with adult periodontitis within the last 2 years, and still presenting with probing depth >5 mm in 2 to 6 teeth, were studied. All subjects were treated with scaling and root planing. Half of the subjects were randomly assigned to receive adjunctive systemic doxycycline (200 mg the Ist day, then 100 mg per day for 21 days). Subgingival plaque samples were taken at baseline, 1, 3 and 6 months after therapy. A modified ELISA test (Evalusite(TM), Periodontal Test Kit, Eastman Kodak Co., Rochester, NY) was used to test for plaque antigens associated with P. gingivalis, P. intermedia and A. actinomycetemcomitans. Progressive alveolar bone loss was determined using digital subtraction radiography with standardized radiographs taken at baseline and 6 months after treatment.Results: The presence of P. gingivalis in plaque after treatment was significantly associated with progressive bone loss (positive predictive value 84%, negative predictive value 85%, odds ratio 31.9, p