High expression of leptin receptor mRNA in breast cancer tissue predicts poor prognosis for patients with high, but not low, serum leptin levels
High expression of leptin receptor mRNA in breast cancer tissue predicts poor prognosis for patients with high, but not low, serum leptin levels
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DOI：
10.1002/ijc.21543
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发表时间：
2006-03-15
影响因子：
6.4
通讯作者：
Noguchi, S
中科院分区：
文献类型：
--
作者：
Miyoshi, Y;Funahashi, T;Noguchi, S
研究了乳腺癌组织中瘦素受体（长亚型：Lep-R（L）和短亚型：Lep-R（S））的mRNA表达水平与患者预后的关系，特别参考了血清瘦素水平或肿瘤组织中瘦素mRNA水平。采用实时荧光定量PCR法检测91例乳腺癌组织中Lep-R（L）、Lep-R（S）和Leptin mRNA水平，采用酶联免疫吸附法检测67例乳腺癌患者血清中Leptin水平。肿瘤组织中Lep-R（L）和Lep-R（S）mRNA水平与患者预后均无显著相关性，但肿瘤内Lep-R（L）和Lep-R（S）mRNA高表达的肿瘤与不良预后显著相关（p < 0.01）。多因素分析显示肿瘤组织中Lep-R（L）和Lep-R（S）mRNA的高水平是显著的危险因素，独立于其他危险因素。亚组分析表明，肿瘤内Lep-R（L）和Lep-R（S）mRNA高表达与高血清瘦素或高肿瘤内瘦素mRNA水平患者亚组的不良预后显著相关，但与低血清瘦素或低肿瘤内瘦素mRNA水平患者亚组无关。肿瘤内Lep-R（L）和Lep-R（S）mRNA高肿瘤与高血清瘦素或高肿瘤内瘦素mRNA水平存在下的不良预后之间的关联似乎表明瘦素和Lep-R（L）/Lep-R（S）途径与乳腺肿瘤的生长刺激有关。肥胖是乳腺癌患者复发的一个危险因素，这一公认的发现可以部分解释为肥胖妇女血清瘦素水平高。(c)2005 Wiley-Liss，Inc.
The association of mRNA expression levels of leptin receptors (long isoform: Lep-R(L) and short isoform: Lep-R(S)) in breast cancer tissue with patient prognosis was studied with special reference to the serum leptin level or the leptin mRNA level in tumor tissue. Lep-R(L), Lep-R(S) and leptin mRNA levels in breast cancer tissue (n = 91) were determined with a real-time PCR assay, and serum leptin levels in breast cancer patients (n = 67) with an enzyme-linked immunosorbent assay. Neither Lep-R(L) nor Lep-R(S) mRNA levels in tumor tissue were significantly associated with patient prognosis, but both intratumoral Lep-R(L) and Lep-R(S) mRNA high tumors were significantly (p < 0.01) associated with a poor prognosis. Multivariate analysis showed that a high level of both Lep-R (L) and Lep-R (S) mRNA in tumor tissue was a significant risk factor, independent of other risk factors. The subset analysis demonstrated that both intratumoral Lep-R(L) and Lep-R(S) mRNA high tumors were significantly associated with a poor prognosis for the subset of patients with high serum leptin or high intratumoral leptin mRNA levels but not in the subset of patients with low serum leptin or low intratumoral leptin mRNA levels. The association between both intratumoral Lep-R(L) and Lep-R(S) mRNA high tumors and a poor prognosis in the presence of high serum leptin or high intratumoral leptin mRNA levels seems to suggest that the leptin and Lep-R(L)l Lep-R(S) pathways are implicated in the growth stimulation of breast tumors. The well-established finding that obesity serves as a risk factor for relapse in breast cancer patients may thus be partially explained by the high serum leptin level seen in obese women. (c) 2005 Wiley-Liss, Inc.