Partial characterization of the embryo-derived platelet-activating factor in mice.
Partial characterization of the embryo-derived platelet-activating factor in mice.
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小鼠胚胎来源的血小板激活因子的部分表征。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
C. O'neill
中科院分区：
文献类型：
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作者：
C. O'neill
小鼠胚胎产生的血小板活化因子（PAF）与1-O-烷基-2-乙酰基-sn-甘油基-3-磷酸胆碱（PAF-acether）的作用动力学和剂量-反应曲线相似。胚胎来源的PAF的活性不受ADP（丙酮酸激酶与磷酸烯醇丙酮酸）或环加氧酶（吲哚美辛）血小板活化途径的抑制剂的影响。氯丙嗪是血小板活化的PAF-乙酰途径的抑制剂，对胚胎来源的PAF的作用有显著的抑制作用。磷脂酶A2，C和D显着抑制活性，而脂肪酶没有影响，表明磷脂结构。所有的胚胎来源的PAF被发现在氯仿：甲醇（2：1 v/v）提取后的氯仿馏分，是PAF-乙酸。两种因子在硅胶薄层色谱（氯仿：甲醇：水;体积比65：35：4）上以相似的速率（Rf 0.10-0.12）迁移。因此，胚胎来源的PAF显示出与PAF-乙酸酯相似的化学、生化和生理特性。
The platelet-activating factor (PAF) produced by mouse embryos showed similar kinetics of action and dose-response curve, in a bioassay, as did 1-0-alkyl-2-acetyl-sn-glyceryl-3-phosphocholine (PAF-acether). The activity of the embryo-derived PAF was not affected by inhibitors of the ADP (pyruvate kinase with phosphoenol pyruvate) or cyclo-oxygenase (indomethacin) pathways of platelet activation. Chlorpromazine, an inhibitor of the PAF-acether pathway of platelet activation, caused a significant inhibition of the effects of embryo-derived PAF. Phospholipases A2, C and D significantly inhibited the activity while lipase had no effect, suggesting a phospholipid structure. All the embryo-derived PAF was found in the chloroform fraction after chloroform:methanol (2:1 v/v) extraction, as was PAF-acether. Both factors migrated at a similar rate (Rf 0.10-0.12) on silica thin-layer chromatography (chloroform:methanol:water; 65:35:4 by vol.). The embryo-derived PAF therefore displays chemical, biochemical and physiological properties similar to those of PAF-acether.