BIOSTIMULATION OF PHOTOSENSITIZED FIBROBLASTS BY LOW INCIDENT LEVELS OF VISIBLE LIGHT ENERGY
BIOSTIMULATION OF PHOTOSENSITIZED FIBROBLASTS BY LOW INCIDENT LEVELS OF VISIBLE LIGHT ENERGY
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低入射水平可见光能量对光敏成纤维细胞的生物刺激
DOI：
10.5978/islsm.95-or-13
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
N. Grossman
中科院分区：
文献类型：
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作者：
R. Lubart;H. Friedmann;M. Sinykov;N. Grossman
长期以来，人们认为可见光（≈380 ~ 650 nm）和近红外（I.R.）（≈650 nm ~ 1000 nm）光不与组织相互作用。然而，在过去的三十年里，人们已经发现，可见光和近红外的激光在非常低的入射能量水平下会对受伤组织产生生物刺激。因此，我们研究了可见光（540 nm, 630 nm,）和近红外（660 nm, 780 nm, 940 nm,）不同光源对成纤维细胞和角质形成细胞的影响。我们发现，在一定的低能量剂量下，它们的增殖增强。由于这两个辐射区域在光化学和光物理性质上有很大的不同，我们认为可见光在被线粒体内的内源性卟啉吸收后开始了代谢事件的级联，而红外辐射通过激活膜上的酶开始了代谢事件的级联。为了验证我们的假设，即可见光下的生物刺激是一个光敏化过程，我们测量了在HeNe （633 nm）照射和940 nm照射后，富含极少量血卟啉衍生物（HPD）的成纤维细胞的增殖率。我们发现HeNe光对成纤维细胞增殖的影响依赖于外源性HPD，而940 nm光对成纤维细胞增殖的影响则不依赖于外源性HPD。这些结果对光疗和光动力治疗（PDT）具有重要的指导意义。
It was long assumed that visible (≈ 380 ∼ 650 nm) and near infrared (I.R.) (≈ 650 nm ∼ 1000 nm) light does not interact with tissue. In the past three decades, however, it has been found that lasers in the visible and near I.R. cause biostimulation of injured tissues at very low levels of incident energy. We therefore studied the effect of various light sources in the visible (540 nm, 630 nm,) and near I.R. (660 nm, 780 nm, 940 nm,) on fibroblasts and keratinocytes. We found that at certain low energy doses there is an enhancement in their proliferation. Since these two radiation regions differ dramatically in their photochemical and photophysical properties, we share the opinion that visible light starts the cascade of metabolic events after being absorbed by endogenous porphyrins in the mitochondria, whereas infrared radiation starts the cascade of metabolic events by activating enzymes in the membranes. In order to examine our hypothesis that biostimulation in the visible is a photosensitization process, we measured the proliferation rate of fibroblasts enriched with very small amounts of hematoporphyrin derivatives (HPD) after HeNe (633 nm) irradiation and 940 nm irradiation. We have found that the effect of HeNe light on fibroblast proliferation was dependent on exogenous HPD while that of 940 nm was not. These results have important implications in both phototherapy and photodynamic therapy (PDT).