III6NeuAcLc4Cer in human SW1116 colorectal carcinoma cells: a possible oncofetal antigen that is not dependent on Lewis gene expression.
III6NeuAcLc4Cer in human SW1116 colorectal carcinoma cells: a possible oncofetal antigen that is not dependent on Lewis gene expression.
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人 SW1116 结直肠癌细胞中的 III6NeuAcLc4Cer：一种可能的癌胚抗原，不依赖于 Lewis 基因表达。
DOI：
10.1016/0003-9861(87)90350-x
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发表时间：
1987
影响因子：
3.9
通讯作者：
Smith,DF
中科院分区：
文献类型：
--
作者：
Law,KL;Smith,DF
用抗人乳唾液低聚糖III6NeuAcLcOse4(唾液四糖)、IV3NeuAcLcOse4(唾液四糖)和IV6NeuAcnLc4Ose(唾液四糖)的单特异性兔抗体检测人结直肠癌细胞SW1116神经节苷脂或神经节苷衍生物唾液酸低聚糖的同源半抗原。III6NeuAcLc4Cer首先在人胎粪中被发现[P.A.Prieto和D.F.Smith(1985)Arch.生物化学。BiPhys.241,281-289]，它存在于SW1116细胞的总神经节苷脂部分中，而不存在于正常人类肠粘膜的总脂提取物中，这与III6NeuAcLc4Cer是一种肿瘤相关的癌胎儿抗原是一致的。NeuAcLc4Cer，人胎粪中的神经节苷脂[P.A.Prieto和D.F.Smith(1986)Arch.生物化学。生物物理学.249,243-253]，也在SW1116细胞中检测到；这一观察结果与它是SW1116细胞中唾液酸-Leaganglioside的直接前体一致。针对唾液酸化的1型寡糖链的特异性抗血清，其表达不依赖于Lewis基因岩藻糖基转移酶，可能是诊断癌胎儿糖类抗原的有用试剂。
Monospecific rabbit antibodies directed against the human milk sialyloligosaccharides III6NeuAcLcOse4(sialyltetrasaccharideb), IV3NeuAcLcOse4(sialyltetrasaccharidea), and IV6NeuAcnLc4Ose (sialyltetrasaccharidec) were used to detect their homologous haptens as gangliosides or ganglioside-derived sialyloligosaccharides from the human colorectal carcinoma cell line SW1116. III6NeuAcLc4Cer was first detected in human meconium [P. A. Prieto and D. F. Smith (1985)Arch. Biochem. Biophys.241,281–289], and its presence in a total ganglioside fraction of SW1116 cells together with its absence from a total lipid extract of normal human intestinal mucosa are consistent with III6NeuAcLc4Cer being a tumor-associated oncofetal antigen. IV3NeuAcLc4Cer, a ganglioside in human meconium [P. A. Prieto and D. F. Smith (1986)Arch. Biochem. Biophys.249,243–253], was also detected in SW1116 cells; an observation that is consistent with its being the immediate precursor to the sialyl-Leaganglioside in SW1116 cells. Specific antisera against sialylated type 1 oligosaccharide chains whose expression is independent of the Lewis gene fucosyltransferase may be useful diagnostic reagents for oncofetal, carbohydrate antigens.