Identifying Novel Phenotypes of Vulnerability and Resistance to Activity-Based Anorexia in Adolescent Female Rats
Identifying Novel Phenotypes of Vulnerability and Resistance to Activity-Based Anorexia in Adolescent Female Rats
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DOI：
10.1002/eat.22149
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发表时间：
2013-11-01
影响因子：
5.5
通讯作者：
Marsteller, Douglas A.
中科院分区：
文献类型：
--
作者：
Barbarich-Marsteller, Nicole C.;Underwood, Mark D.;Marsteller, Douglas A.
基于活动的厌食症是一种转化的啮齿动物模型，导致严重的体重减轻，多动和自愿的自我饥饿。我们研究的目标是识别青春期雌性大鼠活动性厌食症的易感和耐药表型。方法将Sprague-Dawley大鼠维持在限制获取食物的条件下（N=64;或无限获取，N=16），直到实验结束，预定义为目标体重减轻30-35%或满足预定义的动物健康标准。非线性混合效应统计模型被用来描述车轮运行行为，时间事件分析被用来评估实验退出，和回归分区算法被用来分类phenotypes.ResultsObjective标准被确定为区分新的表型的活动为基础的厌食症，包括一个脆弱的表型，赋予最大的多动症，最小的食物摄入量，和最短的时间实验退出，和抗性表型，赋予最小的活动和最长的时间实验exit.DiscussionThe识别的客观标准，用于定义脆弱和抵抗的表型活动为基础的厌食症在青春期雌性大鼠提供了一个重要的框架，研究神经机制，促进脆弱性或保护对发展的自我饥饿和多动症在青春期。最终，未来的研究使用这些新的表型可能会提供重要的翻译的机制，促进这些适应不良的行为特征的神经性厌食症的见解。(c)2013 Wiley Periodicals，Inc. (Int J Eat Disord 2013; 46：737-746）
ObjectiveActivity-based anorexia is a translational rodent model that results in severe weight loss, hyperactivity, and voluntary self-starvation. The goal of our investigation was to identify vulnerable and resistant phenotypes of activity-based anorexia in adolescent female rats.MethodSprague-Dawley rats were maintained under conditions of restricted access to food (N=64; or unlimited access, N=16) until experimental exit, predefined as a target weight loss of 30-35% or meeting predefined criteria for animal health. Nonlinear mixed effects statistical modeling was used to describe wheel running behavior, time to event analysis was used to assess experimental exit, and a regressive partitioning algorithm was used to classify phenotypes.ResultsObjective criteria were identified for distinguishing novel phenotypes of activity-based anorexia, including a vulnerable phenotype that conferred maximal hyperactivity, minimal food intake, and the shortest time to experimental exit, and a resistant phenotype that conferred minimal activity and the longest time to experimental exit.DiscussionThe identification of objective criteria for defining vulnerable and resistant phenotypes of activity-based anorexia in adolescent female rats provides an important framework for studying the neural mechanisms that promote vulnerability to or protection against the development of self-starvation and hyperactivity during adolescence. Ultimately, future studies using these novel phenotypes may provide important translational insights into the mechanisms that promote these maladaptive behaviors characteristic of anorexia nervosa. (c) 2013 Wiley Periodicals, Inc. (Int J Eat Disord 2013; 46:737-746)