Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development.
Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development.
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DOI：
10.1093/hmg/ddp512
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发表时间：
2010-02-01
影响因子：
3.5
通讯作者：
Umezawa A
中科院分区：
文献类型：
--
作者：
Yamada M;Hamatani T;Akutsu H;Chikazawa N;Kuji N;Yoshimura Y;Umezawa A
挖掘基因表达谱数据发现了一种在植入前胚胎中特异性表达的新基因。Hmgpi是一种假定的染色体蛋白，具有两个高迁移率族盒，在合子基因组激活期间合子转录，但在植入后不转录。HMGPI编码的蛋白（HMGPI），首先在4-细胞阶段检测到，仍然在植入前胚胎中高度表达。有趣的是，HMGPI表达在内细胞团（ICM）和滋养外胚层，并从细胞质易位到细胞核在囊胚阶段，表明差异的空间要求之前和之后的囊胚阶段。siRNA（siHmgpi）诱导的Hmgpi转录水平的降低导致植入前胚胎的发育损失和植入失败。此外，Hmgpi的减少阻止胚泡生长，导致胚胎干细胞的产生。注射siHmgpi的胚胎也失去了ICM和滋养外胚层的完整性，其特征是Oct 4、Nanog和Cdx 2的表达减少。这些发现暗示了Hmgpi在哺乳动物胚胎发育的早期阶段的重要作用。
Mining gene-expression-profiling data identified a novel gene that is specifically expressed in preimplantation embryos. Hmgpi, a putative chromosomal protein with two high-mobility-group boxes, is zygotically transcribed during zygotic genome activation, but is not transcribed postimplantation. The Hmgpi-encoded protein (HMGPI), first detected at the 4-cell stage, remains highly expressed in pre-implantation embryos. Interestingly, HMGPI is expressed in both the inner cell mass (ICM) and the trophectoderm, and translocated from cytoplasm to nuclei at the blastocyst stage, indicating differential spatial requirements before and after the blastocyst stage. siRNA (siHmgpi)-induced reduction of Hmgpi transcript levels caused developmental loss of preimplantation embryos and implantation failures. Furthermore, reduction of Hmgpi prevented blastocyst outgrowth leading to generation of embryonic stem cells. The siHmgpi-injected embryos also lost ICM and trophectoderm integrity, demarcated by reduced expressions of Oct4, Nanog and Cdx2. The findings implicated an important role for Hmgpi at the earliest stages of mammalian embryonic development.
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影响因子：
64.5
作者：
Mitsui, K;Tokuzawa, Y;Yamanaka, S
通讯作者：
Yamanaka, S
影响因子：
9.2
作者：
Payer, B;Saitou, M;Surani, MA
通讯作者：
Surani, MA
DOI：
10.1083/jcb.137.1.19
发表时间：
1997-04-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Falciola L;Spada F;Calogero S;Langst G;Voit R;Grummt I;Bianchi ME
通讯作者：
Bianchi ME
影响因子：
4.6
作者：
Levine, AJ;Brivanlou, AH
通讯作者：
Brivanlou, AH
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M