Bioaccumulation of estrogenic hormones and UV-filters in red swamp crayfish (Procambarus clarkii)
Bioaccumulation of estrogenic hormones and UV-filters in red swamp crayfish (Procambarus clarkii)
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DOI：
10.1016/j.scitotenv.2020.142871
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发表时间：
2021-04-10
影响因子：
9.8
通讯作者：
Blaney, Lee
中科院分区：
文献类型：
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作者：
He, Ke;Hain, Ethan;Blaney, Lee
雌激素和有机紫外线过滤剂作为内分泌干扰物（EDCs），由于其强雌激素性和广泛存在于环境中，引起了越来越多的关注。研究了克氏原螯虾体内3种雌激素和5种紫外线过滤剂的积累规律。暴露实验用两种环境相关设计浓度的EDC 5的混合物进行42天（即，500和5000 ng L-1）。水相EDC浓度随时间降低，每两天重新建立一次。在14天的曝光，五个紫外线过滤器测量在2.2至265纳克克（-1）（干重）的小龙虾尾巴组织。在小龙虾中只检测到一种雌激素，17 β-雌二醇，含量为10.4-13.5 ng g（-1）。在接下来的4周暴露期间，未观察到尾部组织中EDC浓度的明显变化。内分泌干扰物的表观生物累积系数从4-甲基亚苄基樟脑的23 L（kg尾组织，干重）（-1）到4-甲氧基肉桂酸2-乙基己酯的1050 L（kg尾组织，干重）（-1）不等。在42天后停止EDC输入，并且更疏水的UV-过滤器（即，三氯异氰尿酸、2-乙基己基-4-甲氧基肉桂酸酯、胡莫柳酯）在整个14天消除期内持续存在。溶胞酶辅助酵母雌激素筛选表明，水样品的残留雌激素活性与（或低于）有针对性的化学分析的预测一致。这些结果表明，转化产物没有显着的雌激素活性，但建议进一步分析内分泌干扰的结果在小龙虾。(C)2020爱思唯尔B. V.保留所有权利。
Estrogenic hormones and organic ultraviolet-filters (UV-filters) have attracted increased attention as endocrine disrupting chemicals (EDCs) due to their potent estrogenicity and widespread occurrence in the environment. This study investigated the accumulation of three estrogenic hormones and five UV-filters in red swamp crayfish (Procambarus clarkii). Exposure experiments were conducted for 42 days with a mixture of EDC5 at two environmentally-relevant design concentrations (i.e., 500 and 5000 ng L-1). The aqueous-phase EDC concentrations decreased over time and were re-established every two days. Within 14 days of exposure, the five UV-filters were measured at 2.2 to 265 ng g(-1) (dry weight) in crayfish tail tissue. Only one estrogenic hormone, 17 beta-estradiol, was detected in the crayfish at 10.4-13.5 ng g(-1) . No apparent changes were observed for EDC concentrations in the tail tissue over the next four weeks of exposure. The apparent bioaccumulation factors for the EDCs ranged from 23 L (kg tail tissue, dry weight)(-1) for 4-methylbenzylidene camphor to 1050 L (kg tail tissue, dry weight)(-1) for 2-ethylhexyl-4-methoxycinnamate. EDC input was stopped after 42 days, and the more hydrophobic UV-filters (i.e., octocrylene, 2-ethylhexyl-4-methoxycinnamate, homosalate) were found to be persistent throughout a 14-d elimination period. A lyticase-assisted yeast estrogen screen demonstrated that the residual estrogenic activity of water samples aligned with (or was lower than) predictions from targeted chemical analysis. These results suggest that the transformation products did not contribute significant estrogenicity, although further analysis of endocrine disruption outcomes in crayfish is recommended. (C) 2020 Elsevier B.V. All rights reserved.