Immunohistochemical detection of nestin in pediatric brain tumors
Immunohistochemical detection of nestin in pediatric brain tumors
复制标题
DOI：
10.1177/002215540205000203
复制
发表时间：
2002-02-01
影响因子：
3.2
通讯作者：
Hendson, G
中科院分区：
文献类型：
--
作者：
Almqvist, PM;Mah, R;Hendson, G
巢蛋白是一种中间丝蛋白（IFP），在中枢神经系统发育过程中的未分化细胞和中枢神经系统肿瘤中表达。关于哪种类型的中枢神经系统肿瘤表达巢蛋白，以往的研究得出了不同的结论。在这份报告中，我们建立了一个免疫组化协议，使用抗原修复，显着增强染色与两个多克隆抗巢蛋白抗血清，#130和#4350。两种巢蛋白抗血清的染色模式是相同的，非常相似的波形蛋白，而胶质细胞酸性蛋白（GFAP），免疫反应是从9.5周龄的前脑缺席。目前的研究20原发性中枢神经系统肿瘤从儿科患者包括7室管膜瘤，7原始神经外胚层肿瘤（PNTR），5毛细胞星形细胞瘤，和一个胶质母细胞瘤多形性（GBM）。所有这些肿瘤表达不同程度的巢蛋白，与五个脑转移瘤测试。强烈的巢蛋白免疫反应被发现在恶性原发性中枢神经系统肿瘤，而良性毛细胞星形细胞瘤表现出低，但一致的巢蛋白表达。在所有肿瘤巢蛋白免疫反应仅限于肿瘤细胞的细胞质，并与星形胶质细胞标记波形蛋白，GFAP，和S-100共表达。所有肿瘤的血管内皮细胞也表现出显着的免疫反应性巢蛋白和波形蛋白，而GFAP和S-100阴性。总之，抗血清#4350检测巢蛋白在福尔马林固定，石蜡包埋的组织切片热诱导抗原修复免疫组化。巢蛋白在高度恶性和低度恶性胶质瘤中均有表达，表明巢蛋白在外科病理学中作为诊断肿瘤标志物的潜在用途。
Nestin is an intermediate filament protein (IFP) expressed in undifferentiated cells during CNS development and in CNS tumors. Previous studies have arrived at different conclusions in terms of which types of CNS tumors express nestin. In this report we establish an immunohistochemical protocol using antigen retrieval, which significantly enhances staining with two polyclonal anti-nestin antisera, #130 and #4350. The staining pattern was identical for the two nestin antisera and very similar to that of vimentin, while glial fibrillary acidic protein (GFAP), immunoreactivity was absent from 9.5-week-old forebrain. The current study of 20 primary CNS tumors from pediatric patients included seven ependymomas, seven primitive neuroectodermal tumors (PNETs), five pilocytic astrocytomas, and one glioblastoma multiforme (GBM). All these tumors expressed nestin to various extents, in contrast to five brain metastases tested. Strong nestin immunoreactivity was found in malignant primary CNS tumors, whereas benign pilocytic astrocytomas showed low but consistent nestin expression. In all tumors nestin immunoreactivity was confined to the cytoplasm of tumor cells and was co-expressed with astrocyte markers vimentin, GFAP, and S-100. Vascular endothelial cells of all neoplasms also showed marked immunoreactivity for nestin and vimentin, whereas they were negative for GFAP and S-100. In conclusion, antiserum #4350 detected nestin in formalin-fixed, paraffin-embedded tissue sections by heat-induced antigen retrieval immunohistochemistry. Nestin was expressed in both highly malignant and low malignant gliomas, indicating the potential use of nestin as a diagnostic tumor marker in surgical pathology.