KU80 - PRODUCT OF THE XRCC5 GENE AND ITS ROLE IN DNA-REPAIR AND V(D)J RECOMBINATION
KU80 - PRODUCT OF THE XRCC5 GENE AND ITS ROLE IN DNA-REPAIR AND V(D)J RECOMBINATION
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DOI：
10.1126/science.8073286
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发表时间：
1994-09-02
期刊：
影响因子：
56.9
通讯作者：
JEGGO, PA
中科院分区：
文献类型：
--
作者：
TACCIOLI, GE;GOTTLIEB, TM;JEGGO, PA
来自中国仓鼠卵巢细胞的辐射敏感突变体XRS-6在DNA双链断裂修复和V(D)J重组能力方面存在缺陷。人类的XRCC5 DNA修复基因，补充了这个突变体，这里通过遗传和生化证据表明，它是Ku蛋白的80千道尔顿亚基。Ku结合到自由的双链DNA末端，是DNA依赖的蛋白激酶的DNA结合成分。因此，Ku蛋白参与DNA修复和V(D)J重组，这些结果也可能表明Ku-DNA依赖的蛋白激酶复合体在这些过程中发挥作用。
The radiosensitive mutant xrs-6, derived from Chinese hamster ovary cells, is defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. The human XRCC5 DNA repair gene, which complements this mutant, is shown here through genetic and biochemical evidence to be the 80-kilodalton subunit of the Ku protein. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase complex in those same processes.