Circadian PER2 protein oscillations do not persist in cycloheximide-treated mouse embryonic fibroblasts in culture
Circadian PER2 protein oscillations do not persist in cycloheximide-treated mouse embryonic fibroblasts in culture
复制标题
经放线菌酮处理的小鼠胚胎成纤维细胞的昼夜节律 PER2 蛋白振荡不会持续存在
DOI：
10.1080/07420528.2017.1316731
复制
发表时间：
2018
影响因子：
2.8
通讯作者：
Okamura Hitoshi
中科院分区：
文献类型：
--
作者：
Tainaka Motomi;Doi Masao;Inoue Yuichi;Murai Iori;Okamura Hitoshi
目前尚不清楚内源性哺乳动物核心时钟蛋白是否在新翻译被抑制的培养细胞中维持可测量的振荡。我们在这里研究了哺乳动物的核心时钟蛋白PER2，它经历了强大的昼夜节律振荡的丰度和磷酸化。与新开发的抗体，使跟踪内源性PER2蛋白振荡的昼夜节律周期与培养的小鼠胚胎成纤维细胞，我们提供的证据表明，PER2不持续明显的昼夜节律时，翻译被抑制。
It is not known whether the endogenous mammalian core clock proteins sustain measurable oscillations in cells in culture wherede novotranslation is pharmacologically inhibited. We studied here the mammalian core clock protein PER2, which undergoes robust circadian oscillations in both abundance and phosphorylation. With a newly developed antibody that enables tracing the endogenous PER2 protein oscillations over circadian cycles with cultured mouse embryonic fibroblast cells, we provide evidence that PER2 does not persist noticeable circadian rhythms when translation is inhibited.