Development of introgression lines derived from Oryza rufipogon and O. glumaepatula in the genetic background of japonica cultivated rice (O. sativa L.) and evaluation of resistance to rice blast
Development of introgression lines derived from Oryza rufipogon and O. glumaepatula in the genetic background of japonica cultivated rice (O. sativa L.) and evaluation of resistance to rice blast
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DOI：
10.1270/jsbbs.60.604
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发表时间：
2010-12-01
期刊：
影响因子：
2.4
通讯作者：
Ando, Ikuo
中科院分区：
文献类型：
--
作者：
Hirabayashi, Hideyuki;Sato, Hiroyuki;Ando, Ikuo
栽培水稻的野生近缘种（Oryza唾液L.）是水稻育种中具有经济价值的等位基因资源。为了有效地鉴定这些等位基因，利用标记辅助回交技术，从具有A基因组的粳稻品种光光（Koshihikari）和Itadaki （Itadaki）的野生近缘中获得了3组携带一个或多个染色体片段的渐近系。供体野生种包括2个稻株（IRGC Acc. 104814和104812）和1个稻株（IRGC Acc. 100968）， 2个粳稻品种光光和Itadaki作为重复亲本。在Koshihikari背景下，两组紫花莲的il分别为40和47行。在104814和104812种质中，亲本复发基因的平均比例分别为94.3%和96.0%。在Itadaki背景下，来自黄颡鱼（O. glumaepatula）的il共有47个系，平均亲本复发基因组比例为91.0%。为了证明这些白介素在鉴定有用等位基因方面的潜力，我们对这两组白介素进行了水稻稻瘟病抗性筛选。通过代入定位，在水稻稻瘟病种质（104812）3号和11号染色体上发现了两个新的稻瘟病部分抗性位点。
Wild relatives of cultivated rice (Oryza saliva L.) are useful sources of alleles with economic value for rice breeding programs. To effectively identify such alleles, three sets of introgression lines (ILs) carrying one or more chromosome segments derived from wild relatives with the A genome in the background of japonica rice cultivars Koshihikari and Itadaki were developed using marker-assisted backcrossing. The donor wild species include two accessions of O. rufipogon (IRGC Acc. 104814 and 104812) and one accession of O. glumaepatula (IRGC Acc. 100968) while 2 japonica rice cultivars, Koshihikari and Itadaki, were used as recurrent parents. The two sets of ILs from O. rufipogon in the Koshihikari background consisted of 40 and 47 lines, respectively. The average proportions of recurrent parental genome were 94.3% and 96.0% in the ILs derived from accessions 104814 and 104812, respectively. The ILs from O. glumaepatula in the Itadaki background consisted of 47 lines, with an average proportion of recurrent parental genome of 91.0%. To demonstrate the potential of these ILs in identifying useful alleles, the two sets of ILs were screened for resistance to rice blast (Magnaporthe grisea). By substitution mapping, two new loci for partial resistance to rice blast were detected on chromosomes 3 and 11 of O. rufipogon (104812).