Carbon and nitrogen limitation of soil microbial respiration in a High Arctic successional glacier foreland near Ny-Ålesund, Svalbard
Carbon and nitrogen limitation of soil microbial respiration in a High Arctic successional glacier foreland near Ny-Ålesund, Svalbard
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DOI：
10.1111/j.1751-8369.2007.00001.x
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发表时间：
2007-01
期刊：
影响因子：
1.9
通讯作者：
S. Yoshitake;M. Uchida;H. Koizumi;T. Nakatsubo
中科院分区：
文献类型：
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作者：
S. Yoshitake;M. Uchida;H. Koizumi;T. Nakatsubo
碳和氮的可用性限制微生物活性的假设，和限制微生物的关键因素变化沿着演替梯度在高北极冰川前陆进行了测试。我们研究了呼吸速率和磷脂脂肪酸含量的反应，除了碳和/或氮。在斯瓦尔巴特群岛的新勒松附近的一个冰川前陆，采集了初级演替早期和晚期的土壤样品。碳（葡萄糖）和氮（硝酸铵）的添加引起的微生物呼吸速率在演替的早期阶段的增加。相反，添加碳或氮并没有增加微生物的呼吸速率。在演替后期，单独添加碳以及同时添加碳和氮都增加了微生物的呼吸速率。然而，在10 ° C下培养15天内，无论是添加碳还是添加氮都不会影响任何土壤的总磷脂脂肪酸含量（微生物生物量指数）。因此，呼吸速率的增加归因于微生物群落的生理活动的变化，例如酶活性。我们的研究表明，微生物呼吸是有限的，在演替的早期阶段的碳和氮的低可用性。此后，氮限制减轻。
The hypotheses that carbon and nitrogen availability limit microbial activity, and that the key factors limiting microbes vary along the successional gradient were tested in a High Arctic glacier foreland. We examined the responses of the respiration rate and the phospholipid fatty acid content to the addition of carbon and/or nitrogen. Soil samples were collected from the early stage and late stage of primary succession in the foreland of a glacier near Ny-Ålesund, Svalbard. The addition of both carbon (glucose) and nitrogen (ammonium nitrate) engendered an increase in the microbial respiration rate in the early stage of succession. In contrast, the addition of either carbon or nitrogen did not increase the microbial respiration rate. In the late stage of succession the addition of carbon alone, as well as the addition of both carbon and nitrogen, increased the microbial respiration rate. However, neither the addition of carbon nor the addition of nitrogen affected the total phospholipid fatty acid content (an index of microbial biomass) for any soil within 15 days of incubation at 10 ° C. An increase in the respiration rate was therefore attributed to changes in the physiological activities of the microbial community, such as enzymatic activity. Our study suggests that microbial respiration was limited by the low availability of both carbon and nitrogen in the early stage of succession. Thereafter, nitrogen limitation is mitigated.