Renal glucose reabsorption during hypertonic glucose infusion in female streptozotocin-induced diabetic rats
Renal glucose reabsorption during hypertonic glucose infusion in female streptozotocin-induced diabetic rats
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DOI：
10.1016/s0024-3205(01)01090-6
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发表时间：
2001-05-18
期刊：
影响因子：
6.1
通讯作者：
Banks, RO
中科院分区：
文献类型：
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作者：
Noonan, WT;Shapiro, VM;Banks, RO
有关糖尿病患者肾脏葡萄糖转运能力的信息有限。这些数据是必要的，因为在注射链脲佐菌素(STZ)两周后，大鼠近端小管葡萄糖转运蛋白GLUT2的mRNA和蛋白水平上调。因此，我们测定了雌性对照组大鼠以及注射STZ后1周(STZ-1)、2周(STZ-2)和3周(STZ-3)(65 mg kg(-1)，ip)的大鼠肾脏葡萄糖转运和GLUT2蛋白水平。将渐进量的葡萄糖通过股静脉注入麻醉大鼠，并收集肾脏清除情况。以肾小球滤过率(GFR)为因素，STZ-3组大鼠的葡萄糖重吸入量显著高于所有其他组。此外，与对照组相比，STZ-1和STZ-2组的葡萄糖重吸收量减少，而STZ-3组的葡萄糖重吸收量增加。相比之下，所有服用STZ的大鼠肾脏GLUT2水平均升高。这些数据表明，与GLUT2共同或独立发挥作用的其他因素也需要支持肾脏葡萄糖转运能力的提高。(C)2001 Elsevier Science Inc.保留所有权利。
Information regarding the renal glucose transport capacity in diabetes mellitus is limited. These data are needed because two weeks following injection of streptozotocin (STZ), mRNA and protein levels of the glucose transporter, GLUT2, are upregulated in the proximal tubule of the rat. Therefore, we measured renal glucose transport and GLUT2 protein levels in female control rats, and in rats one (STZ-1), two (STZ-2), and three weeks (STZ-3) after STZ injection (65 mg kg(-1), ip). Progressive amounts of glucose were infused into anesthetized rats via the femoral Vein and renal clearances collected. The amount of glucose reabsorbed, factored by the glomerular filtration rate (GFR) was significantly greater in STZ-3 rats compared with all other groups. In addition, the amount of glucose reabsorbed factored by the amount of glucose filtered was decreased in STZ-1 and STZ-2 compared with controls but was increased in STZ-3. By contrast, renal GLUT2 levels were elevated in all the STZ-treated rats. These data suggest that other factors, functioning either in conjunction with or independent of GLUT2, are required to support an elevated renal glucose transport capacity. (C) 2001 Elsevier Science Inc. All rights reserved.