Heterogeneity of circulating CXCR5-PD-1^hiTph cells in patients of type 2 and type 1 diabetes in Chinese population
Heterogeneity of circulating CXCR5-PD-1^hiTph cells in patients of type 2 and type 1 diabetes in Chinese population
复制标题
DOI：
10.1007/s00592-023-02055-6
复制
发表时间：
2023-03
影响因子：
3.8
通讯作者：
Zhangyao Su;Chenggong Ma;Ruiling Zhao;Yin-Zhen Jiang;Yun Cai;Gu Yong;Tao Yang;Xinyu Xu
中科院分区：
文献类型：
--
作者：
Zhangyao Su;Chenggong Ma;Ruiling Zhao;Yin-Zhen Jiang;Yun Cai;Gu Yong;Tao Yang;Xinyu Xu
循环外周辅助性T （Tph）细胞可促进自身免疫性疾病的进展。然而，Tph细胞在炎性疾病（如2型糖尿病（T2DM））中的作用以及T2DM与自身免疫性糖尿病之间的差异尚不清楚。方法选取T2DM患者92例，T1DM患者106例，健康对照84例。分离外周血单核细胞，采用多色流式细胞术检测。我们进一步评估了循环Tph细胞与临床生化参数、胰岛功能、疾病进展和胰岛自身抗体之间的相关性。结果T2DM和T1DM患者外周血Tph细胞均明显高于正常对照组。T2DM患者和超重T2DM患者的Tph细胞和B细胞呈显著正相关。T2DM患者Tph细胞与c肽曲线下面积（C-PAUC）呈负相关，Tph细胞与空腹血糖、糖化血红蛋白水平呈显著正相关。但在T1DM患者中，Tph细胞与上述临床指标均无相关性。T1DM患者Tph细胞频率与GAD自身抗体滴度和病程呈正相关。此外，我们证明了T1DM患者接受利妥昔单抗治疗后，Tph细胞的频率降低。结论T2DM患者外周血Tph细胞与血糖水平及胰岛功能相关。在T1DM患者中，循环Tph细胞与B细胞和胰岛自身抗体相关。这可能提示Tph细胞在两种糖尿病中具有不同的致病机制。临床试验信息http://clinicaltrials .gov NCT01280682（注册于2010年7月）。
AimsCirculating peripheral helper T (Tph) cells are shown to promote the progression of autoimmune diseases. However, the role of Tph cells in inflammatory diseases such as type 2 diabetes mellitus (T2DM) and the differences between T2DM and autoimmune diabetes remain unclear.MethodsWe recruited 92 T2DM patients, 106 type 1 diabetes mellitus (T1DM) patients and 84 healthy control individuals. Peripheral blood mononucleated cells were isolated and examined by multicolor flow cytometry. We further evaluated the correlations between circulating Tph cells and clinical biochemical parameters, islet function, disease progression and islet autoantibodies.ResultsCirculating Tph cells were significantly higher in both T2DM and T1DM patients than in healthy control individuals. A significant positive correlation was observed between Tph cells and B cells in T1DM patients and overweight T2DM patients. Furthermore, Tph cells were negatively correlated with the area under the C-peptide curve (C-PAUC), and Tph cells were significantly positively correlated with fasting glucose and glycated hemoglobin levels in T2DM patients. However, no correlation was found between Tph cells and the above clinical indicators in T1DM patients. The frequency of Tph cells positively correlated with the titer of GAD autoantibodies and duration of disease in T1DM patients. In addition, we demonstrated that the frequency of Tph cells was decreased after rituximab therapy in T1DM patients.ConclusionsCirculating Tph cells are associated with blood glucose levels and islet function in T2DM patients. In T1DM patients, circulating Tph cells are associated with B cells and islet autoantibodies. This may suggest that Tph cells have different pathogenic mechanisms in the two types of diabetes.Clinical trial informationhttp://ClinicalTrials.gov NCT01280682 (registered July, 2010).