Fluorescent Labeling of Yeast Cell Wall Components.
Fluorescent Labeling of Yeast Cell Wall Components.
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DOI：
10.1101/pdb.prot085241
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发表时间：
2016-08
影响因子：
--
通讯作者：
Hiroki Okada;Y. Ohya
中科院分区：
文献类型：
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作者：
Hiroki Okada;Y. Ohya
可以在荧光显微镜下观察用与细胞壁组分之一特异性结合的荧光染料染色的酵母细胞。组分1，3-β-葡聚糖、甘露糖蛋白和/或几丁质的可视化不仅提供了关于细胞壁的信息，而且还揭示了关于各种细胞活动的线索，例如细胞极性、囊泡运输、出芽模式的建立、顶端和各向同性芽生长以及复制细胞年龄。该协议描述了一个标准的方法可视化酵母细胞壁的不同组成部分。
Yeast cells stained with a fluorescent dye that specifically binds to one of the cell wall components can be observed under a fluorescent microscope. Visualization of the components 1,3-β-glucan, mannoproteins, and/or chitin not only provides information concerning the cell wall, but also reveals clues about various cellular activities such as cell polarity, vesicular transport, establishment of budding pattern, apical and isotropic bud growth, and replicative cell age. This protocol describes a standard method for visualizing different components of the yeast cell wall.