The impact of aging, hearing loss, and body weight on mouse hippocampal redox state, measured in brain slices using fluorescence imaging.
The impact of aging, hearing loss, and body weight on mouse hippocampal redox state, measured in brain slices using fluorescence imaging.
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DOI：
10.1016/j.neurobiolaging.2016.03.006
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发表时间：
2016-06
影响因子：
4.2
通讯作者：
Llano DA
中科院分区：
文献类型：
--
作者：
Stebbings KA;Choi HW;Ravindra A;Llano DA
海马体中的氧化应激与其他衰老相关变化（如听力损失、皮质变薄或体重变化）之间的关系尚不清楚。我们测量了年轻和老年小鼠脑切片中许多神经结构的氧化还原比。还测量了听力阈值、体重和皮质厚度。我们发现显着的老化相关的氧化还原比的增加，被隔离到皮层，而这样的变化没有观察到丘脑或皮质。这些变化主要是由FAD而不是NADH的变化驱动的。多元回归分析表明，听力阈值和皮层厚度都没有独立的海马氧化还原比的变化。然而，体重没有独立的预测海马氧化还原比的变化。这些数据表明，海马氧化还原比的衰老相关变化不是整体脑氧化状态的一般反映，而是高度局部化的，同时仍然与至少一种晚期衰老的标志物有关，即生命结束时的体重减轻。
The relationships between oxidative stress in the hippocampus and other aging-related changes such as hearing loss, cortical thinning or changes in body weight are not yet known. We measured the redox ratio in a number of neural structures in brain slices taken from young and aged mice. Hearing thresholds, body weight and cortical thickness were also measured. We found striking aging-related increases in the redox ratio that were isolated to the stratum pyramidale, while such changes were not observed in thalamus or cortex. These changes were driven primarily by changes in FAD, not NADH. Multiple regression analysis suggested that neither hearing threshold nor cortical thickness independently contributed to this change in hippocampal redox ratio. However, body weight did independently contribute to predicted changes in hippocampal redox ratio. These data suggest that aging-related changes in hippocampal redox ratio are not a general reflection of overall brain oxidative state, but are highly localized, while still being related to at least one marker of late aging, weight loss at the end of life.