Filter matrix estimation in automated DNA sequencing.
Filter matrix estimation in automated DNA sequencing.
复制标题
自动 DNA 测序中的过滤矩阵估计。
DOI：
10.1109/10.664198
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
States,DJ
中科院分区：
文献类型：
--
作者：
Huang,W;Fuhrmann,DR;Politte,DG;ThomasJr,LJ;States,DJ
In four-color fluorescence-based automated DNA sequencing, a 4/spl times/4 filter matrix parameterizes the relationship between the dye-intensity signals of interest and the data collected by an optical imaging system. The filter matrix is important because the estimated DNA sequence is based on the dye intensities that can only be recovered via inversion of the matrix. Here, the authors present a calibration method for the estimation of the columns of this matrix, using data generated through a special experiment in which DNA samples are labeled with only one fluorescent dye at a time. Simulations and applications of the method to real data are provided, with promising results.
