Resistance to lambda-cyhalothrin in Spanish field populations of Ceratitis capitata and metabolic resistance mediated by P450 in a resistant strain
Resistance to lambda-cyhalothrin in Spanish field populations of Ceratitis capitata and metabolic resistance mediated by P450 in a resistant strain
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DOI：
10.1002/ps.3924
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发表时间：
2015-09-01
影响因子：
4.1
通讯作者：
Ortego, Felix
中科院分区：
文献类型：
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作者：
Arouri, Rabeh;Le Goff, Gaelle;Ortego, Felix
欧盟于2009年撤销马拉硫磷，导致用于控制西班牙柑橘作物中地中海实蝇Ceratitis capitata的三氟氯氰菊酯应用量大幅增加。Capitata对三氟氯氰菊酯产生了抗药性（6-14倍），达到的LC 50值（129-287 ppm）高于田间处理的推荐浓度（125 ppm）。这些结果与高敏感性，三氟氯氰菊酯在突尼斯现场人群中发现。我们研究了实验室筛选的抗性菌株W-1 K（205倍抗性）的抗性机制。增效剂的生物测定表明，抗性几乎完全被P450抑制剂PBO抑制。本研究对CYP 4、CYP 6、CYP 9和CYP 12家族的53个P450基因在C. capitata的结果显示，CYP 6A 51在抗性菌株中过表达（13-18倍）。W-1 K菌株对醚菊酯（240倍）和溴氰菊酯（150倍）也表现出高水平的交互抗性。capitata。P450介导的代谢抗性是W-1 K菌株的主要抗性机制。发现的交叉抗性水平可能会损害其他拟除虫菊酯控制该物种的有效性。(c)2014年化学工业协会
BACKGROUNDThe withdrawal of malathion in the European Union in 2009 resulted in a large increase in lambda-cyhalothrin applications for the control of the Mediterranean fruit fly, Ceratitis capitata, in Spanish citrus crops.RESULTSSpanish field populations of C. capitata have developed resistance to lambda-cyhalothrin (6-14-fold), achieving LC50 values (129-287 ppm) higher than the recommended concentration for field treatments (125 ppm). These results contrast with the high susceptibility to lambda-cyhalothrin found in three Tunisian field populations. We have studied the mechanism of resistance in the laboratory-selected resistant strain W-1K (205-fold resistance). Bioassays with synergists showed that resistance was almost completely suppressed by the P450 inhibitor PBO. The study of the expression of 53 P450 genes belonging to the CYP4, CYP6, CYP9 and CYP12 families in C. capitata revealed that CYP6A51 was overexpressed (13-18-fold) in the resistant strain. The W-1K strain also showed high levels of cross-resistance to etofenprox (240-fold) and deltamethrin (150-fold).CONCLUSIONField-evolved resistance to lambda-cyhalothrin has been found in C. capitata. Metabolic resistance mediated by P450 appears to be the main resistance mechanism in the resistant strain W-1K. The levels of cross-resistance found may compromise the effectiveness of other pyrethroids for the control of this species. (c) 2014 Society of Chemical Industry