Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana
Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana
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DOI：
10.1074/jbc.m307552200
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发表时间：
2003-11-28
影响因子：
4.8
通讯作者：
Adam, G
中科院分区：
文献类型：
--
作者：
Poppenberger, B;Berthiller, F;Adam, G
镰刀菌属的植物病原真菌会引起小粒谷物和玉米等重要的农业病害。毛霉烯是由不同的镰刀菌产生的一类真菌毒素，它能抑制真核生物蛋白质的生物合成，并可能干扰植物防御反应过程中诱导的基因表达。其成员之一脱氧雪腐镰刀菌烯醇很可能是真菌致病过程中的毒力因子，并经常在谷物中积累到威胁人类和动物健康的水平。我们从拟南芥中分离并鉴定了一个编码脱氧雪腐镰刀菌烯醇的UDP糖基转移酶基因。该酶以前被命名为UGT73C5，催化葡萄糖从UDP-葡萄糖转移到脱氧雪腐镰刀菌烯醇的第三碳基羟基。使用小麦胚芽提取物耦合的转录/翻译系统，我们已经证明了这种酶反应灭活了真菌毒素。该脱氧雪腐镰刀菌烯醇葡萄糖基转移酶(DOGT1)对乙酰化衍生物15-乙酰脱氧雪腐镰刀菌烯醇也有解毒作用，但对结构相似的雪腐镰刀菌烯醇没有保护作用。糖基转移酶的表达受脱氧雪腐镰刀菌烯醇以及水杨酸、乙烯和茉莉酸的发育调节和诱导。在拟南芥中的结构性过表达导致对脱氧雪腐镰刀菌烯醇的耐受性增强。
Plant pathogenic fungi of the genus Fusarium cause agriculturally important diseases of small grain cereals and maize. Trichothecenes are a class of mycotoxins produced by different Fusarium species that inhibit eukaryotic protein biosynthesis and presumably interfere with the expression of genes induced during the defense response of the plants. One of its members, deoxynivalenol, most likely acts as a virulence factor during fungal pathogenesis and frequently accumulates in grain to levels posing a threat to human and animal health. We report the isolation and characterization of a gene from Arabidopsis thaliana encoding a UDP-glycosyltransferase that is able to detoxify deoxynivalenol. The enzyme, previously assigned the identifier UGT73C5, catalyzes the transfer of glucose from UDP-glucose to the hydroxyl group at carbon 3 of deoxynivalenol. Using a wheat germ extract-coupled transcription/ translation system we have shown that this enzymatic reaction inactivates the mycotoxin. This deoxynivalenol-glucosyltransferase (DOGT1) was also found to detoxify the acetylated derivative 15-acetyl-deoxynivalenol, whereas no protective activity was observed against the structurally similar nivalenol. Expression of the glucosyltransferase is developmentally regulated and induced by deoxynivalenol as well as salicylic acid, ethylene, and jasmonic acid. Constitutive overexpression in Arabidopsis leads to enhanced tolerance against deoxynivalenol.