Reproductive Induction is a Far-Red High Irradiance Response that is Mediated by Phytochrome and PHYTOCHROME INTERACTING FACTOR in Marchantia polymorpha
Reproductive Induction is a Far-Red High Irradiance Response that is Mediated by Phytochrome and PHYTOCHROME INTERACTING FACTOR in Marchantia polymorpha
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DOI：
10.1093/pcp/pcz029
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发表时间：
2019-05-01
影响因子：
4.9
通讯作者：
Kohchi, Takayuki
中科院分区：
文献类型：
--
作者：
Inoue, Keisuke;Nishihama, Ryuichi;Kohchi, Takayuki
陆地植物进化出一系列光感受器来精确地感知环境信息。其中，光敏色素是红光（R）和远红光（FR）的唯一光感受器，在调节各种发育过程中发挥着关键作用。大多数现存的陆生植物具有多种光敏色素，这些光敏色素可能是从陆生植物共同祖先的单一光敏色素进化而来的。然而，祖先光敏色素信号转导机制仍然是未知的，由于缺乏知识，光敏色素的功能，在基地植物。最近有报道，Mpphy，一个单一的光敏色素在肝麦芽地钱多形物，调节典型的光可逆反应统称为低通量反应（LFR）。在这里，我们表明，Mpphy也调节配子体的形成类似于被子植物中的远红外高辐照度响应（FR-HIR）的作用模式。我们使用通过基于CRISPR/ Cas9的基因组编辑获得的突变体植物进行的表型分析显示，MpFHY 1（远红伸长下胚轴1的直系同源物）以及Mpphy对于M中的FR-HIR信号传导至关重要。多形。此外，敲除M. polymorpha，完全废除了FR-HIR依赖的配子体形成，而MpPIF的过表达加速了反应。在Mppif突变体中，FR-HIR依赖性转录调控也被破坏。我们的研究结果表明，植物已经获得了FR-HIR信号介导的光敏色素和PIF在陆地植物进化过程中的非常早期的阶段，和一个单一的光敏色素在陆地植物的共同祖先可以介导LFR和FR-HIR。
Land plants have evolved a series of photoreceptors to precisely perceive environmental information. Among these, phytochromes are the sole photoreceptors for red light (R) and far-red light (FR), and play pivotal roles in modulating various developmental processes. Most extant land plants possess multiple phytochromes that probably evolved from a single phytochrome in the common ancestor of land plants. However, the ancestral phytochrome signaling mechanism remains unknown due to a paucity of knowledge regarding phytochrome functions in basal land plants. It has recently been reported that Mpphy, a single phytochrome in the liver-wort Marchantia polymorpha, regulates typical photoreversible responses collectively classified as low fluence response (LFR). Here, we show that Mpphy also regulates the gametangiophore formation analogous to the mode of action of the far-red high irradiance response (FR-HIR) in angiosperms. Our phenotypic analyses using mutant plants obtained by CRISPR/ Cas9-based genome editing revealed that MpFHY1, an ortholog of FAR-RED ELONGATED HYPOCOTYL1, as well as Mpphy is critical for the FR-HIR signaling in M. polymorpha. In addition, knockout of MpPIF, a single PHYTOCHROME INTERACTING FACTOR gene in M. polymorpha, completely abolished the FR-HIR-dependent gametangiophore formation, while overexpression of MpPIF accelerated the response. FR-HIR-dependent transcriptional regulation was also disrupted in the Mppif mutant. Our findings suggest that plants had already acquired the FR-HIR signaling mediated by phytochrome and PIF at a very early stage during the course of land plant evolution, and that a single phytochrome in the common ancestor of land plants could mediate both LFR and FR-HIR.