Monoclonal antibody analysis of human T lymphocyte subpopulations exhibiting autologous mixed lymphocyte reaction.
Monoclonal antibody analysis of human T lymphocyte subpopulations exhibiting autologous mixed lymphocyte reaction.
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对表现出自体混合淋巴细胞反应的人 T 淋巴细胞亚群进行单克隆抗体分析。
DOI：
10.1073/pnas.78.8.5096
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发表时间：
1981
影响因子：
11.1
通讯作者：
Gupta,S
中科院分区：
文献类型：
--
作者：
Damle,NK;Hansen,JA;Good,RA;Gupta,S
在自体混合淋巴细胞反应（MLR）中，T细胞在自体非T细胞的刺激下增殖。在本研究中，用小鼠单克隆抗体OKT4、OKT8或okt9.3定义的人T细胞亚群，检测了它们在自体和异体MLR中的增殖能力。我们观察到应答T细胞用OKT4或9.3抗体（定义辅助/诱导T细胞）和补体（C’）处理后，它们在自体MLR中的增殖反应减弱，并显著降低其异体MLR的增殖反应。相比之下，用OKT8抗体（定义抑制/细胞毒性T细胞）和C'处理T细胞对其在自体MLR中的增殖反应没有或只有很小的影响。此外，OKT4, 9.3, 9.6 （PAN）或7.2抗人Ia（框架特异性）但OKT8抗体，当在整个培养期间存在时，在没有C'的情况下，以剂量依赖的方式抑制自体和异体MLR的增殖反应。用OKT4或9.3抗体预处理应答T细胞，清洗，然后用辐照的自体或异体非T细胞刺激，也观察到增殖抑制。在缺乏C′的情况下，用OKT8或7.2抗ia抗体预处理T细胞不影响其在自体MLR中的增殖反应。因此，含有OKT4或9.3抗体定义的辅助/诱导剂活性细胞的T细胞似乎是自体MLR的主要应答T细胞亚群。
In autologous mixed lymphocyte reaction (MLR), T cells proliferate in response to the stimulation by autologous non-T cells. In the present study, human T cell subpopulations, defined by murine monoclonal antibodies OKT4, OKT8, or 9.3, were examined for their capacity to proliferate in autologous and allogeneic MLR. It was observed that the treatment of responder T cells with OKT4 or 9.3 antibody (both defining helper/inducer T cells) and complement (C') ablated their proliferative response in autologous MLR and markedly reduced their allogeneic MLR proliferative response. In contrast, treatment of T cells with OKT8 antibody (defining suppressor/cytotoxic T cells) and C' had no or minimal effect on their proliferative response in autologous MLR. Furthermore, OKT4, 9.3, 9.6 (PAN), or 7.2 anti-human Ia (framework specific) but OKT8 antibody, when present during the entire culture period, in the absence of C' inhibited in a dose-dependent manner the proliferative response in both autologous and allogeneic MLR. Inhibition of proliferation was also observed when the responder T cells were pretreated with OKT4 or 9.3 antibody, washed, and then stimulated with irradiated autologous or allogeneic non-T cells. Pretreatment of T cells with OKT8 or 7.2 anti-Ia antibody in the absence of C' did not influence their proliferative response in autologous MLR. Thus, T cells containing cells with helper/inducer activity defined by OKT4 or 9.3 antibody appear to be the major responder T-cell subpopulation in autologous MLR.