Reversible DNA i-motif to hairpin switching induced by copper(II) cations.
Reversible DNA i-motif to hairpin switching induced by copper(II) cations.
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DOI：
10.1039/c5cc05111h
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发表时间：
2015-09-25
期刊：
影响因子：
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通讯作者：
Waller ZA
中科院分区：
文献类型：
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作者：
Day HA;Wright EP;MacDonald CJ;Gates AJ;Waller ZA
形成DNA序列的I基序以前曾被用于许多不同的纳米技术应用，但都是利用pH的变化来折叠DNA。结果表明，在不改变pH值的情况下，铜(II)离子可用于将I-基序重新折叠成发夹结构。I-Motif DNA结构以前曾被用于许多不同的纳米技术应用，但都利用pH的变化来折叠DNA。在这里，我们描述了铜(II)离子如何改变I-基序DNA的构象，使其成为可通过与EDTA螯合而可逆的发夹结构。
i-Motif forming DNA sequences have previously been used for many different nanotechnological applications, but all have used changes in pH to fold the DNA. Here it is shown that Cu(ii) cations can be used to re-fold i-motifs into hairpin structures, without changing the pH. i-Motif DNA structures have previously been utilised for many different nanotechnological applications, but all have used changes in pH to fold the DNA. Herein we describe how copper(ii) cations can alter the conformation of i-motif DNA into an alternative hairpin structure which is reversible by chelation with EDTA.