Morphological transformation and phosphatidylserine exposure in erythrocytes treated with ribavirin.
Morphological transformation and phosphatidylserine exposure in erythrocytes treated with ribavirin.
复制标题
用利巴韦林处理的红细胞的形态转变和磷脂酰丝氨酸暴露。
DOI：
--
复制
发表时间：
2009
影响因子：
2
通讯作者：
Y. Kohda
中科院分区：
文献类型：
--
作者：
M. Homma;Hiroyuki Hosono;Y. Hasegawa;Y. Kohda
研究了利巴韦林对人红细胞中磷脂酰丝氨酸（PS）形态和再分布的影响。红细胞用1mm利巴韦林孵育，在有/没有双嘧达莫（es型核苷转运蛋白抑制剂）的情况下孵育。细胞内利巴韦林在红细胞中积累，浓度为1361 muM，与接受利巴韦林治疗的患者血药浓度一致。双嘧达莫通过抑制红细胞es型核苷转运蛋白，使利巴韦林积累减少40.7% （807 muM）。利巴韦林治疗后，86.4%的红细胞形态转化为棘细胞。双嘧达莫预处理使形态学变化降低20.0%。与对照组相比，利巴韦林增加了暴露ps的细胞（2.15%比0.87%）。双嘧达莫抑制利巴韦林的积累也减少了ps暴露细胞（0.62%）。结果提示细胞内利巴韦林诱导红细胞形态改变和PS暴露，加速网状内皮系统的红细胞吞噬。
The effects of intracellular ribavirin on morphology and redistribution of phosphatidylserine (PS) in human erythrocytes were examined. Erythrocytes were incubated with 1 mM ribavirin in the presence/absence of dipyridamole, an inhibitor of es-type nucleoside transporter. Intracellular ribavirin was accumulated in erythrocytes with the concentration of 1361 muM, which corresponds to the blood level in patients receiving ribavirin. Dipyridamole reduced ribavirin accumulation by 40.7% (807 muM) via inhibiting es-type nucleoside transporter on erythrocytes. Morphological transformation into echinocytic form was observed in 86.4% of the erythrocytes treated with ribavirin. Dipyridamole pre-treatment decreased the morphological change to 20.0%. Ribavirin increased the PS-exposing cells compared with control (2.15% vs. 0.87%). PS-exposing cells were also decreased by inhibiting ribavirin accumulation with dipyridamole (0.62%). The results suggest that intracellular ribavirin induces morphological change and PS exposure in erythrocytes and accelerates erythrophagocytosis in the reticuloendothelial system.
影响因子：
158.5
作者：
McHutchison, JG;Gordon, SC;Albrecht, JK
通讯作者：
Albrecht, JK
影响因子：
20.3
作者：
Kuypers, FA;Yuan, J;Schrier, SL
通讯作者：
Schrier, SL