Increased expression of matrix metalloproteinase-9 in the eutopic endometrial tissue of women with endometriosis
Increased expression of matrix metalloproteinase-9 in the eutopic endometrial tissue of women with endometriosis
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DOI：
10.1093/humrep/del297
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发表时间：
2006-12-01
影响因子：
6.1
通讯作者：
Akoum, A.
中科院分区：
文献类型：
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作者：
Collette, T.;Maheux, R.;Akoum, A.
背景：子宫内膜异位症是一种子宫内膜组织异位植入的疾病。细胞外基质（ECM）的重塑是该组织植入的先决条件。方法：采用正交试验设计在这项研究中，我们检测免疫反应性基质金属蛋白酶-9（MMP-9）在整个子宫内膜组织，并确定血管性血友病因子（vWF）阳性内皮细胞，CD 45阳性白细胞，CD 3阳性T淋巴细胞和CD 68阳性巨噬细胞作为细胞表达MMP-9的基质。研究结果：酶谱法和酶联免疫吸附试验（ELISA）检测结果显示，MMP-9在子宫内膜异位症患者子宫内膜组织中的表达明显增高（P < 0.05）。然而，RT-PCR没有显示这些组织中MMP-9 mRNA表达的统计学显著增加（P = 0.14）。有和没有子宫内膜异位症的妇女之间没有显着差异的表达组织抑制剂MMP（TIMP）-1，一个已知的天然抑制剂的前和活性形式的MMP-9，无论是通过ELISA或RT-PCR检测（P = 0.46和0.37，分别）。MMP-9/TIMP-1在子宫内膜异位症患者子宫内膜中的蛋白表达和mRNA表达均显著高于正常对照组（P < 0.05）。结论：这些发现使得MMP-9/TIMP-1失衡参与子宫内膜异位症患者子宫内膜组织的侵袭性和疾病的异位发展成为可能。
BACKGROUND: Endometriosis is a disease where endometrial tissue implants in ectopic locations. Remodelling of the extracellular matrix (ECM) is a prerequisite for the implantation of this tissue to be possible. METHODS: In this study, we detected immunoreactive matrix metalloproteinase-9 (MMP-9) throughout endometrial tissue and identified von Willebrand factor (vWF)-positive endothelial cells, CD45-positive leukocytes, CD3-positive T lymphocytes and CD68-positive macrophages as cells expressing MMP-9 in the stroma. RESULTS: We found an increased expression of MMP-9 in the uterine endometrial tissue of women with endometriosis, as assessed by zymography and enzyme-linked immunosorbent assay (ELISA) (P < 0.05). However, RT-PCR did not show a statistically significant increase in MMP-9 mRNA expression in these tissues (P = 0.14). There was no significant difference between women with and without endometriosis in the expression of tissue inhibitor of MMPs (TIMP)-1, a known natural inhibitor of the pro- and active forms of MMP-9, whether tested by ELISA or by RT-PCR (P = 0.46 and 0.37, respectively). Interestingly, the ratio of MMP-9/TIMP-1 expression was significantly higher in women with endometriosis than in normal women both at the protein and the mRNA levels (P < 0.05). CONCLUSION: These findings make plausible the involvement of MMP-9/TIMP-1 imbalance in the invasiveness of the endometrial tissue of patients with endometriosis and the ectopic development of the disease.