Variegated expression of the Sgs-4 locus in Drosophila melanogaster.
Variegated expression of the Sgs-4 locus in Drosophila melanogaster.
复制标题
果蝇中 Sgs-4 基因座的多样化表达。
DOI：
10.1007/bf00288495
复制
发表时间：
1986
期刊：
影响因子：
1.6
通讯作者：
Kauffman,SA
中科院分区：
文献类型：
--
作者：
Kornher,JS;Kauffman,SA
对(1；4)wm258-21只黑腹果蝇幼虫的实验证实了唾液腺位置效应变异的特异性标记的存在。该标记是一种名为Sgs-4的胶蛋白基因，在三龄幼虫期间表达。使用温度作为杂化调节剂，我们发现Sgs-4染色体基因座的细胞学致密性在17℃时增强，在29℃时减弱。我们还发现，在17℃时唾液腺中的Sgs-4蛋白和转录本积累到29℃时唾液腺中Sgs-4蛋白和转录本的大约一半。Southern分析表明，Sgs-4基因座在17℃时被多角化到29℃时水平的大约三分之一。
Experiments onT(1;4)wm258-21larvae ofDrosophila melanogasterare described which establish the existence of a salivary gland specific marker for position-effect variegation. The marker is a glue protein gene calledSgs-4which is expressed during the third larval instar. Using temperature as a variegation modifier, we showed that cytological compaction for theSgs-4chromosomal locus is enhanced at 17° C and reduced at 29° C. We also found that theSgs-4protein and transcript from salivary glands at 17° C accumulate to roughly half the levels found in salivary glands at 29° C. Southern analysis suggested that theSgs-4locus at 17° C is polytenized to roughly one-third the level at 29° C. The results are discussed with respect to alternative models of variegation.