Fibroblast growth factor, but not activin, is a potent activator of mitogen-activated protein kinase in Xenopus explants.
Fibroblast growth factor, but not activin, is a potent activator of mitogen-activated protein kinase in Xenopus explants.
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成纤维细胞生长因子（但不是激活素）是爪蟾外植体中丝裂原激活蛋白激酶的有效激活剂。
DOI：
10.1073/pnas.91.5.1662
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发表时间：
1994
影响因子：
11.1
通讯作者：
Kimelman,D
中科院分区：
文献类型：
--
作者：
Graves,LM;Northrop,JL;Potts,BC;Krebs,EG;Kimelman,D
从非洲爪蟾胚胎的动物半球分离的外植体与非洲爪蟾碱性成纤维细胞生长因子（XbFGF）或人激活素A孵育。XbFGF孵育导致促分裂原活化蛋白激酶（MAPK）和核糖体S6蛋白激酶（pp 90 rsk）以剂量依赖性方式快速活化，最高活化水平发生在50 ng/ml。最大激活发生在6-10分钟后，添加生长因子，和两种激酶的活性下降到未刺激的水平后30分钟。激活素是无法激活MAPK或pp 90 rsk在非洲爪蟾外植体的实质性水平，虽然它诱导背中胚层比XbFGF在相同的实验条件下。调节蛋白Xwnt-8不激活MAPK，也不增强XbFGF对MAPK的激活。XbFGF能够激活MAPK通过至少midgastrula阶段，这表明这个家庭的生长因子可能有一个原肠胚阶段的事件中的作用。
Isolated explants from the animal hemisphere of Xenopus embryos were incubated with Xenopus basic fibroblast growth factor (XbFGF) or human activin A. XbFGF incubation resulted in the rapid activation of mitogen-activated protein kinase (MAPK) and ribosomal S6 protein kinase (pp90rsk) in a dose-dependent manner with the highest levels of activation occurring at 50 ng/ml. Maximal activation occurred within 6-10 min after the addition of growth factor, and the activity of both kinases declined to unstimulated levels after 30 min. Activin was unable to activate either MAPK or pp90rsk in the Xenopus explants to a substantial level, although it induced dorsal mesoderm better than XbFGF under the same experimental conditions. The regulatory protein Xwnt-8 did not activate MAPK, nor did it enhance the activation of MAPK by XbFGF. XbFGF was able to activate MAPK through at least the midgastrula stage, suggesting that this family of growth factors may have a role in gastrula-stage events.