Community dynamics and the lower airway microbiota in stable chronic obstructive pulmonary disease, smokers and healthy non-smokers
Community dynamics and the lower airway microbiota in stable chronic obstructive pulmonary disease, smokers and healthy non-smokers
复制标题
DOI：
10.1136/thoraxjnl-2015-207235
复制
发表时间：
2016-09-01
期刊：
影响因子：
10
通讯作者：
Elborn, J. S.
中科院分区：
文献类型：
--
作者：
Einarsson, G. G.;Comer, D. M.;Elborn, J. S.
近年来，细菌在COPD进展中的作用越来越受到重视。然而，在COPD患者的下呼吸道的微生物群落的复杂性是很差的characteristic.Objectives比较COPD患者，吸烟者和非smoking.Methods的下呼吸道微生物群从成人COPD（n=18），吸烟者没有气道疾病（n=8）和健康人（n=11）的支气管冲洗样本进行了分析，通过扩展培养和培养的非依赖性Illumina MiSeq测序。我们确定了需氧和厌氧微生物群负荷，并评估了与三个队列相关的细菌差异。使用非培养分析来确定比较组之间的微生物群差异，包括分类丰富度、多样性、相对丰度、核心微生物群和共存性。测量和主要结果扩展培养显示三个队列之间的需氧菌和厌氧菌的总负荷没有差异。非培养分析显示，假单胞菌属成员的患病率。在COPD患者的下呼吸道中更高;然而，该分类群的大部分序列读数归因于三名患者。此外，拟杆菌门的成员，如普雷沃氏菌属，观察到健康“比较组”中的差异更大。与健康群体相比，COPD患者的社区多样性（和）明显较少。共同出现的细菌类群和观察一个假定的核心'社区内的下airways.Conclusions COPD患者的下气道中的微生物群落组成是显着不同的吸烟者和非吸烟者中发现的，表明该疾病的一个组成部分是与微生物状态的变化。
Rationale The role bacteria play in the progression of COPD has increasingly been highlighted in recent years. However, the microbial community complexity in the lower airways of patients with COPD is poorly characterised.Objectives To compare the lower airway microbiota in patients with COPD, smokers and non-smokers.Methods Bronchial wash samples from adults with COPD (n=18), smokers with no airways disease (n=8) and healthy individuals (n=11) were analysed by extended-culture and culture-independent Illumina MiSeq sequencing. We determined aerobic and anaerobic microbiota load and evaluated differences in bacteria associated with the three cohorts. Culture-independent analysis was used to determine differences in microbiota between comparison groups including taxonomic richness, diversity, relative abundance, core' microbiota and co-occurrence.Measurement and main results Extended-culture showed no difference in total load of aerobic and anaerobic bacteria between the three cohorts. Culture-independent analysis revealed that the prevalence of members of Pseudomonas spp. was greater in the lower airways of patients with COPD; however, the majority of the sequence reads for this taxa were attributed to three patients. Furthermore, members of Bacteroidetes, such as Prevotella spp., were observed to be greater in the healthy' comparison groups. Community diversity ( and ) was significantly less in COPD compared with healthy groups. Co-occurrence of bacterial taxa and the observation of a putative core' community within the lower airways were also observed.Conclusions Microbial community composition in the lower airways of patients with COPD is significantly different to that found in smokers and non-smokers, indicating that a component of the disease is associated with changes in microbiological status.