The interaction between amylose and amylopectin synthesis in rice endosperm grown at high temperature
The interaction between amylose and amylopectin synthesis in rice endosperm grown at high temperature
复制标题
高温生长的水稻胚乳中直链淀粉和支链淀粉合成的相互作用
DOI：
10.1016/j.foodchem.2019.125258
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发表时间：
2019-12-15
期刊：
影响因子：
8.8
通讯作者：
Liu, Qiaoquan
中科院分区：
文献类型：
--
作者：
Fan, Xiaolei;Li, Yingqiu;Liu, Qiaoquan
淀粉是稻米胚乳中含量丰富的组分，其微观结构决定着稻米的品质和功能特性。灌浆期高温对淀粉的微细结构有显著影响。然而，对温度升高条件下淀粉合成相关酶之间的竞争以及直链淀粉和支链淀粉生物合成之间的相互作用还知之甚少。本研究以非糯和糯水稻为材料，在常温和高温条件下进行种植试验。利用数学模型对淀粉微观结构进行参数化分析，结果表明：直链淀粉的合成与支链淀粉长链（DP > 60）的伸长竞争;支链淀粉短链可以作为支链淀粉长链伸长的底物;高温消除了直链淀粉和支链淀粉合成的一致性和规律性。此外，酶活性测定结果也验证了淀粉数学建模分析拟合结果的有效性。
Starch is the abundant component in rice endosperm, and its microstructure determines the quality and functional properties of rice grain. It is well known that the starch fine structure is markedly influenced by high temperature during grain filling. However, it is poorly understood on the competition among starch synthesis related enzymes as well as the interaction between amylose and amylopectin biosynthesis under increased growing temperature. In this study, the non-waxy and waxy rice were planted under normal and high temperatures. Parameterizing analysis of the starch microstructure using mathematical models proved that amylose synthesis competed with the elongation of long amylopectin chains (DP > 60); Short chains of amylopectin can be used as the substrate for elongation of longer amylopectin chains; High temperature eliminated the consistency and regularly of the synthesis of amylose and amylopectin. In addition, enzyme assay proved the validly of fitting results from mathematical modeling analysis of starch.