Two isoforms of sarco/endoplasmic reticulum calcium ATPase (SERCA) are essential in Caenorhabditis elegans
Two isoforms of sarco/endoplasmic reticulum calcium ATPase (SERCA) are essential in Caenorhabditis elegans
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DOI：
10.1016/s0378-1119(00)00536-9
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发表时间：
2000-12-31
期刊：
影响因子：
3.5
通讯作者：
Ahnn, J
中科院分区：
文献类型：
--
作者：
Cho, JH;Bandyopadhyay, J;Ahnn, J
肌浆网/内质网钙ATP酶（Sarco/Endoplasmic Reticulum Calcium ATPase，SERCA）是一种膜结合的Ca ~（2+）/Mg ~（2+）依赖性ATP酶，能将Ca ~（2+）螯合到SR/ER腔中，是维持细胞内Ca ~（2+）稳态的重要成分之一。在这里，我们描述了一个C的鉴定和功能表征。elegans SERCA基因（ser-1）。Ser-1是在其羧基末端选择性剪接以形成两种同种型（SER-1A和SER-1B）的单基因，并且显示出与哺乳动物SERCA的高度同源性（70%同一性，80%相似性）。绿色荧光蛋白（green fluorescent protein，GFP）和整体包埋免疫组化分析显示，SER-1在神经元细胞、体壁肌、咽肌和外阴肌、排泄细胞和外阴上皮细胞中均有表达。此外，SER-1：：GFP在胚胎阶段表达，并且表达在成体阶段维持。针对每种SER-I亚型的双链RNA注射（也称为RNAi）导致严重的表型缺陷：ser-IA（RNAi）动物显示胚胎致死，而ser-IB（RNAi）导致L1幼虫停滞表型。这些结果表明，这两种亚型的C。与哺乳动物一样，秀丽线虫SERCA对胚胎发育和胚后生长和存活至关重要。(C)2000 Elsevier Science B. V.保留所有权利。
SERCA (Sarco/Endoplasmic Reticulum Calcium ATPase), a membrane bound Ca2+-/Mg2+- dependent ATPase that sequesters Ca2+ into the SR/ER lumen, is one of the essential components for the maintenance of intracellular Ca2+ homeostasis. Here we describe the identification and functional characterization of a C. elegans SERCA gene (ser-1). ser-l is a single gene alternatively spliced at its carboxyl terminus to form two isoforms (SER-1A and SER-1B) and displays a high homology (70% identity, 80% similarity) with mammalian SERCAs. Green fluorescent protein (GFP) and whole-mount immunostaining analyses reveal that SER-1 expresses in neuronal cells, body wall muscles, pharyngeal and vulval muscles, excretory cells, and vulva epithelial cells. Furthermore, SER-1::GFP expresses during embryonic stages and the expression is maintained through the adult stages. Double-stranded RNA injection (also known as RNAi) targeted to each SER-I isoform results in severe phenotypic defects: ser-IA(RNAi) animals show embryonic lethality, whereas ser-IB(RNAi) results in L1 larval arrest phenotype. These findings suggest that both isoforms of C. elegans SERCA, like in mammals, are essential for embryonic development and post-embryonic growth and survival. (C) 2000 Elsevier Science B.V. All rights reserved.