Cell therapy in murine atherosclerosis: in vivo imaging with high-resolution helical SPECT.
Cell therapy in murine atherosclerosis: in vivo imaging with high-resolution helical SPECT.
复制标题
小鼠动脉粥样硬化的细胞疗法：高分辨率螺旋 SPECT 体内成像。
DOI：
10.1148/radiol.2421051461
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发表时间：
2007
期刊：
影响因子：
19.7
通讯作者：
Chin,BennettB
中科院分区：
文献类型：
--
作者：
Vemulapalli,Sreekanth;Metzler,ScottD;Akabani,Gamal;Petry,NeilA;Niehaus,NelsenJ;Liu,Xialin;Patil,NikhilH;Greer,KimL;Jaszczak,RonaldJ;Coleman,REdward;Dong,Chunming;Goldschmidt-Clermont,PascalJ;Chin,BennettB
目的：利用高分辨率全身螺旋单光子发射计算机断层扫描（SPECT）技术，确定铟111 (111In)-氧标记的骨髓（BM）在小鼠动脉粥样硬化和血管修复模型中的体内定位和定量的可行性。材料和方法：机构动物护理和使用委员会批准了本研究。年轻的B6 Rosa 26 Lac Z+/+小鼠BM用111in -oxine放射性标记。在给予放射性标记细胞后的第1、4和7天，用全身高分辨率螺旋SPECT对5只C57/BL6载脂蛋白e缺陷小鼠和5只野生型（WT）对照小鼠进行成像。用SPECT定量与用伽马计数离体分析进行比较。放射自显影和β-半乳糖苷酶染色证实供体细胞的生物分布。采用线性回归评估连续变量之间的相关性。双尾学生检验用于比较组间值，双尾配对检验用于评估受试者在不同时间点的变化。结果：SPECT图像对比度高，放射标记细胞给药后7天BM，肝脏和脾脏清晰可见。SPECT显示载脂蛋白e缺陷小鼠与WT小鼠相比，主动脉和BM部位的活性分别高出42%和58%（两者的P< 0.05）。此外，载脂蛋白e缺乏小鼠的肝脏和脾脏的活性分别比WT小鼠低28%和27%（两者的P< 0.05）。SPECT与器官γ计数有良好的定量相关性（r= 0.9）。受体主动脉的β-半乳糖苷酶染色和显微放射自显像显示供体细胞定位于可见的动脉粥样硬化斑块内膜，但未定位于血管壁的未受影响区域。结论：高分辨率体内螺旋针孔SPECT可用于监测和量化111in -oxine标记的BM在动脉粥样硬化祖细胞治疗小鼠模型中的早期生物分布。©rsna, 2007
Purpose:To determine the feasibility of in vivo localization and quantification of indium 111 (111In)-oxine–labeled bone marrow (BM) with high-resolution whole-body helical single photon emission computed tomography (SPECT) in an established murine model of atherosclerosis and vascular repair.Materials and Methods:The institutional animal care and use committee approved this study. BM from young B6 Rosa 26 Lac Z+/+mice was radiolabeled with111In-oxine. On days 1, 4, and 7 after administration of radiolabeled cells, five C57/BL6 apolipoprotein E–deficient mice and five wild-type (WT) control mice were imaged with whole-body high-resolution helical SPECT. Quantification with SPECT was compared with ex vivo analysis by means of gamma counting. Autoradiography and β-galactosidase staining were used to verify donor cell biodistribution. Linear regression was used to assess the correlation between continuous variables. Two-tailed Studentttest was used to compare values between groups, and paired two-tailedttest was used to assess changes within subjects at different time points.Results:SPECT image contrast was high, with clear visualization of BM, liver, and spleen 7 days after administration of radiolabeled cells. SPECT revealed that 42% and 58% more activity was localized to the aorta and BM (P< .05 for both), respectively, in apolipoprotein E–deficient mice versus WT mice. Furthermore, 28% and 27% less activity was localized to the liver and spleen (P< .05 for both), respectively, in apolipoprotein E–deficient mice versus WT mice. SPECT and organ gamma counts showed good quantitative correlation (r= 0.9). β-Galactosidase staining and microautoradiography of recipient aortas showed donor cell localization to the intima of visible atherosclerotic plaque but not to unaffected regions of the vessel wall.Conclusion:High-resolution in vivo helical pinhole SPECT can be used to monitor and quantify early biodistribution of111In-oxine–labeled BM in a murine model of progenitor cell therapy for atherosclerosis.© RSNA, 2007