A comparative study of preventing postoperative tendon adhesion using electrospun polyester membranes with different degradation kinetics
A comparative study of preventing postoperative tendon adhesion using electrospun polyester membranes with different degradation kinetics
复制标题
不同降解动力学静电纺聚酯膜预防术后肌腱粘连的比较研究
DOI：
10.1007/s11426-015-5425-7
复制
发表时间：
2015-07-01
影响因子：
9.6
通讯作者：
Chen, Xuesi
中科院分区：
文献类型：
--
作者：
Song, Zhiming;Shi, Bo;Chen, Xuesi
肌腱损伤引起的并发症包括腱鞘感染和腱周粘连，其中肌腱粘连常常导致严重的运动功能障碍。在这项工作中，使用具有不同降解动力学的聚（L-丙交酯）（PLA）和聚（ε-己内酯）（PCL）的电纺膜来研究它们在跟腱修复中的抗粘连功效。与 PCL 膜相比，PLA 样品在 42 d 内表现出更快的降解速度，并且所有降解介质（即磷酸盐缓冲盐水）均保持在 7.4 左右的恒定 pH 值。同时，体外细胞粘附试验和体内组织病理学检测证明了PLA和PCL膜均具有优异的生物相容性。同时，PLA 膜在降低粘附力和促进功能恢复方面比 PCL 样品更有效。此外，苏木精-伊红和Masson三色染色以及I型胶原免疫组化分析进一步证实了实验结果。所有结果表明，静电纺PLA膜处理的模型在抗粘连和肌腱修复方面均明显优于PCL膜组。考虑到降解和防粘连功效的结果，静电纺聚酯膜，尤其是PLA膜，在临床预防术后肌腱粘连方面将具有巨大的应用潜力。
Complications arising from tendon injury include tendon sheath infection and peritendinous adhesion, in which tendon adhesion often leads to serious motor dysfunction. In this work, the electrospun membranes of poly(L-lactide) (PLA) and poly(epsilon-caprolactone) (PCL) with different degradation kinetics were used to investigate their efficacy for anti-adhesion toward Achilles tendon repair. Compared with the PCL membrane, the PLA sample showed a faster rate of degradation in 42 d, and all the degradation media (i.e., phosphate-buffered saline) maintained at a constant pH of around 7.4. Meanwhile, the superior biocompatibility of both the PLA and PCL membranes were proved by the in vitro cellular adhesion tests and in vivo histopathological assays. Simultaneously, the PLA membrane was more effective than the PCL sample in decreasing adhesion and promoting functional recovery. Furthermore, the experiment result was further confirmed by hematoxylin-eosin and Masson's trichrome staining, and type I collagen immunohistochemical analysis. All results revealed that the model treated with the electrospun PLA membrane was obviously better with regard to both anti-adhesion and tendon repair than that in the PCL membrane group. Considering the results of degradation and adhesion prevention efficacy, the electrospun polyester membranes, especially the PLA one, would be applied with fascinating potential in clinical prevention of postoperative tendon adhesion.