tRNA Actively Shuttles Between the Nucleus and Cytosol in Yeast
tRNA Actively Shuttles Between the Nucleus and Cytosol in Yeast
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DOI：
10.1126/science.1113346
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发表时间：
2005-07
期刊：
影响因子：
56.9
通讯作者：
A. Takano;T. Endo;T. Yoshihisa
中科院分区：
文献类型：
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作者：
A. Takano;T. Endo;T. Yoshihisa
以往的证据表明，转移RNA(TRNAs)在细胞核中成熟后只穿过核膜一次进入胞浆。我们现在提出了酵母中tRNAs核进口的证据。一些出口突变体即使在没有转录的情况下也会在细胞核中积累成熟的tRNA。进口需要能源，但不需要RAN周期。这些结果表明，tRNAs穿梭于细胞核和胞浆之间。
Previous evidence suggested that transfer RNAs (tRNAs) cross the nuclear envelope to the cytosol only once after maturing in the nucleus. We now present evidence for nuclear import of tRNAs in yeast. Several export mutants accumulate mature tRNAs in the nucleus even in the absence of transcription. Import requires energy but not the Ran cycle. These results indicate that tRNAs shuttle between the nucleus and cytosol.