In vivo experimental study of nanosecond pulsed electric field effects on solid tumors
In vivo experimental study of nanosecond pulsed electric field effects on solid tumors
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DOI：
10.1109/tdei.2013.6571443
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发表时间：
2013-08
影响因子：
3.1
通讯作者：
M. Nagahama;N. Shimomura;A. Nakagawa;K. Teranishi;Y. Uto;H. Hori
中科院分区：
文献类型：
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作者：
M. Nagahama;N. Shimomura;A. Nakagawa;K. Teranishi;Y. Uto;H. Hori
许多研究已经测试了脉冲电场在癌症治疗期间对肿瘤的应用。在这项研究中，我们使用鸡胚试验来测试纳秒脉冲电场（nsPEFs）对实体瘤的影响。我们的实验使用鸡胚试验，这被归类为体内实验，因为它有几个优点。使用小鼠乳腺癌细胞系EMT6/KU来产生用于实验的肿瘤细胞。在测试前将细胞接种数天，并使用针电极将nsPEF施加到在卵的绒毛尿囊膜上形成的实体瘤。实验中使用了脉冲输出长度为1.5 ns的脉冲功率发生器。在nsPEF应用几天后从卵中解剖肿瘤并称重。用脉冲处理的肿瘤的平均重量小于对照。nsPEF应用后肿瘤重量与对照组之间的差异显著。随着脉冲功率发生器的充电电压的增加，差异增大，当脉冲功率发生器的充电电压> 2.85 kV时观察到显著差异。在CAM上或CAM下很少发生放电的组的平均肿瘤重量显著小于未发生放电的组。
Many studies have tested the application of pulsed electric fields to tumors during cancer therapy. In this study, we used an embryonic chick assay to test the effects of nanosecond pulsed electric fields (nsPEFs) on a solid tumor. Our experiments used the embryonic chick assay, which is classified as in vivo experiment, because it has several advantages. Mouse breast adenocarcinoma cell line EMT6/KU was used to produce tumor cells for the experiment. The cells were inoculated for several days before the test and nsPEFs were applied using needle electrodes to solid tumors formed on the chorioallantoic membrane of eggs. A pulsed power generator with a pulse output length of 1.5 ns was used in this experiment. The tumors were dissected from the eggs after several days of nsPEF application and weighed. The mean weight of tumors treated with pulses was less than that of the control. The difference between the weight of the tumor after nsPEF application and that of the control was significant. The difference increased with the charging voltage of the pulsed power generator, with significant different observed when the charging voltage of the pulsed power generator was >2.85 kV. The mean tumor weight of the group that experienced discharges infrequently occurred on or under CAM was significantly smaller than that of the group that did not experience.