Initiation of genetic recombination and recombination-dependent replication
Initiation of genetic recombination and recombination-dependent replication
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DOI：
10.1016/s0968-0004(00)01569-3
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发表时间：
2000-04-01
影响因子：
13.8
通讯作者：
Kowalczykowski, SC
中科院分区：
文献类型：
--
作者：
Kowalczykowski, SC
重组始于双链DNA断裂和单链DNA间隙。当DNA聚合酶遇到DNA模板或其他蛋白质的缺陷时，这些DNA链的不连续性可以由DNA损伤剂和正常的DNA复制引起。同源重组机制在这些断裂和间隙处起作用，促进导致基因重组的事件，以及分离复制臂的重新附着和DNA复制的恢复。在大肠杆菌中，这些事件需要协作（RecA、RecBCD、RecFOR、RecQ、RuvABC和SSB蛋白）和DNA复制（PriABC蛋白和DNA聚合酶）。回顾了这些重组和重组依赖复制过程的共同初始步骤。
Recombination initiates at double-stranded DNA breaks and at single-stranded DNA gaps. These DNA strand discontinuities can arise from DNA-damaging agents and from normal DNA replication when the DNA polymerase encounters an imperfection in the DNA template or another protein. The machinery of homologous recombination acts at these breaks and gaps to promote the events that result in gene recombination, as well as the reattachment of detached replication arms and the resumption of DNA replication. In Escherichia coli, these events require collaboration (RecA, RecBCD, RecFOR, RecQ, RuvABC and SSB proteins) and DNA replication (PriABC proteins and the DNA polymerases). The initial steps common to these recombination and recombination-dependent replication processes are reviewed.