Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination.
Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination.
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DOI：
10.1007/978-1-4939-7862-5_11
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Nishiyama A
中科院分区：
文献类型：
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作者：
Sherafat A;Hill RA;Nishiyama A
少突胶质细胞发育和髓鞘形成是中枢神经系统中受细胞内在和外在机制调节的过程。器官型切片培养提供了一个简单的方法，研究因素，影响少突胶质细胞增殖，分化和髓鞘形成的背景下，当地的细胞环境。在这里，我们表明，主要的胶质细胞类型和神经元保存在切片培养从出生后的小鼠前脑，其形态特征保留。我们进一步证明，需要与邻近细胞相互作用的细胞过程，如髓鞘形成，可以在切片培养中进行。
Oligodendrocyte development and myelination are processes in the central nervous system that are regulated by cell intrinsic and extrinsic mechanisms. Organotypic slice cultures provide a simple method for studying factors that affect oligodendrocyte proliferation, differentiation, and myelination in the context of the local cellular environment. Here we show that major glial cell types and neurons are preserved in slice cultures from postnatal mouse forebrain, and their morphological characteristics are retained. We further demonstrate that cellular processes requiring interactions with neighboring cells such as myelination can proceed in slice culture.