Stabilization of glutathione in urine and plasma: relevance to urinary metal excretion studies.
Stabilization of glutathione in urine and plasma: relevance to urinary metal excretion studies.
复制标题
尿液和血浆中谷胱甘肽的稳定性：与尿液金属排泄研究的相关性。
DOI：
10.1093/jat/9.1.31
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发表时间：
1985
影响因子：
2.5
通讯作者：
Kostyniak,PJ
中科院分区：
文献类型：
--
作者：
Mulder,KM;Kostyniak,PJ
目前的研究证明了谷胱甘肽（GS）在尿液和血浆中的不稳定性，并说明了开发程序以稳定生物体液中GS的重要性。用L-（αS，5S）-α-氨基-3-氯-4，5-二氢-5-异恶唑乙酸（AT-125）、EDTA或柠檬酸钠抑制γ-谷氨酰转肽酶（−-GTP）不能阻止尿GSH的快速丢失。通过在采集后立即酸化样品，可使尿液中的GS稳定较长时间。为了准确测量尿液和血浆GS浓度，通过已知的添加技术分析样品。这些分析方法的金属排泄研究的相关性进行了讨论。
The present studies demonstrate the instability of glutathione (GS) in urine and plasma and illustrate the importance of developing procedures to stabilize GS in biological fluids. The rapid loss of urinary GSH was not prevented by inhibition of y-glutamyltranspeptidas (−-GTP) with L-(αS,5S)-α-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (AT-125), by EDTA, or by sodium citrate. GS in urine could be stabilized for extended periods of time by acidification of samples immediately after collection. In order to accurately measure urine and plasma GS concentrations, samples were analyzed by a known additions technique. The relevance of these analytical procedures to metal excretion studies is discussed.