BRAIN-STEM PROJECTIONS OF SENSORY AND MOTOR COMPONENTS OF THE VAGUS NERVE IN THE RAT
BRAIN-STEM PROJECTIONS OF SENSORY AND MOTOR COMPONENTS OF THE VAGUS NERVE IN THE RAT
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DOI：
10.1002/cne.902110304
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发表时间：
1982-01-01
影响因子：
2.5
通讯作者：
SULLIVAN, JM
中科院分区：
文献类型：
--
作者：
KALIA, M;SULLIVAN, JM
用辣根过氧化物酶（HRP）神经组织化学方法，对16只雄性Wistar大鼠颈迷走神经及其下神经节（结状神经节）在延髓和上颈髓的感觉和运动联系进行了追踪。使用四甲基联苯胺（TMB）作为底物的HRP允许的可视化transganglionic和逆行运输的感觉神经末梢和胞体，分别。大鼠的迷走神经以许多束进入延髓，进入点覆盖延髓的整个外侧，从头尾侧+4至-6 mm水平延伸至奥贝。迷走神经感觉纤维束进入延髓背外侧，并行进至孤束（TS），其在延髓中标记为> 8.8mm。接受迷走神经投射的TS的尾侧范围位于颈髓（C1-C2）的V层。感觉终末野可见于双侧孤束核（NTS）、最后区（AP）和迷走神经运动背核（DMNX）。同侧NTS和DMNX的投影比对侧重。两侧的最后区被强烈标记。来自DMNX中HRP标记的胞体的运动纤维以不同的纤维束向腹内侧行进，随后再分成许多小纤维束，这些小纤维束以HRP标记纤维的精细网络的形式穿过髓质网状结构。当这些来自DMNX的纤维接近延髓的腹外侧时，它们由来自疑核（NA）、疑后核（NRA）和面后核（NRF）的轴突连接。这些后一种纤维在网状结构的中间形成发夹环，以伴随来自DMNX的轴突在腹外侧位置从髓质离开。HRP标记的胞体，在NTS中的感觉末梢中的transganglionically运输的HRP相反，只在一侧可视化，从而表明通过迷走神经的运动控制仅由位于同侧的运动神经元施加。另一方面，感觉信息分散到位于髓质两侧的许多核亚群。
The sensory and motor connections of the cervical vagus nerves and of its inferior ganglion (nodose ganglion) were traced in the medulla and upper cervical spinal cord of 16 male Wistar rats by using horseradish peroxidase (HRP) neurohistochemistry. The use of tetramethyl benzidine (TMB) as the substrate for HRP permitted the visualization of transganglionic and retrograde transport in sensory nerve terminals and perikarya, respectively. The vagus nerve in the rat enters the medulla in numerous fascicles with points of entry covering the entire lateral aspect of the medulla extending from level +4 to -6 mm rostrocaudal to the obex. Fascicles of vagal sensory fibers enter the dorsolateral aspect of the medulla and travel to the tractus solitarius (TS) which was labeled for > 8.8 mm in the medulla. The caudal extent of the TS receiving vagal projections was found in lamina V of the cervical spinal cord (C1-C2). Sensory terminal fields could be visualized bilaterally in the nucleus of the tractus solitarius (NTS), area postrema (AP) and dorsal motor nucleus of the vagus nerve (DMNX). The ipsilateral projection to the NTS and the DMNX was heavier than that found on the contralateral side. The area postrema was intensely labeled on both sides. Motor fibers from HRP-labeled perikarya in the DMNX travel ventromedially in a distinct fascicle and subsequently subdivide into a number of small fiber bundles that traverse the medullary reticular formation in the form of the fine network of HRP-labeled fibers. As these fibers from the DMNX approach the ventrolateral aspect of the medulla, they are joined by axons from the nucleus ambiguus (NA), nucleus retroambigualis (NRA) and the retrofacial nucleus (NRF). These latter fibers form hairpin loops in the middle of the reticular formation to accompany the axons from the DMNX exiting from the medulla in a ventrolateral location. HRP-labeled perikarya, in contrast to transganglionically transported HRP in sensory terminals in the NTS, were visualized on one side only, thus indicating that motor control via the vagus nerve is exerted only by motor neurons located ipsilaterally. Sensory information on the other hand diverges to many nuclear subgroups located on both sides of the medulla.