Bacteriologic diagnosis of nosocomial pneumonia in primates. Usefulness of the protected specimen brush.
Bacteriologic diagnosis of nosocomial pneumonia in primates. Usefulness of the protected specimen brush.
复制标题
灵长类动物医院肺炎的细菌学诊断。
DOI：
10.1164/arrd.1982.125.1.53
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
JohansonJr,WG
中科院分区：
文献类型：
--
作者：
Higuchi,JH;Coalson,JJ;JohansonJr,WG
在油酸诱导的急性弥漫性肺损伤和自然发生的“院内肺炎”的灵长类动物模型中，我们评估了保护标本刷(PSB)在获取未受污染的下呼吸道材料进行细菌学检查方面的有用性。每一次肺炎的细菌病因都是通过立即死后肺抽吸和/或死前血培养来确定的。在研究的15只动物中，有12只发生了细菌性肺炎。经胸片检查，11只经气管插管的正常狒狒和7只有非肺炎浸润性病变的动物的PSB培养均为无菌。在每一种情况下，PSB都避免了标本受到近端呼吸道菌群的污染。在10只经细菌学证实为肺炎的狒狒中，PSB培养正确地鉴定了7只动物的病原体，尽管X线片显示弥漫性肺浸润物和近端呼吸道分泌物中存在多种致病菌。在这些动物中，10个PSB样本中只有1个(10%)被可能无关的病原体污染。PSB在很大程度上避免了培养标本受到近端呼吸道菌群的污染，因此在区分呼吸道细菌定植和弥漫性肺浸润性肺炎方面应该是有用的。
We evaluated the usefulness of a protected specimen brush (PSB) in obtaining uncontaminated lower respiratory tract material for bacteriologic examination in a primate model of oleic acid-induced acute diffuse lung injury and naturally occurring “nosocomial pneumonia.” The bacterial cause of each pneumonia was established by either an immediate postmortem lung aspirate and/or antemortem blood culture. Bacterial pneumonia occurred in 12 of the 15 animals studied. The PSB cultures were sterile in 11 normal, intubated baboons and in 7 animals with nonpneumonic infiltrates by chest radiography. In each of these instances contamination of the specimen by proximal airway flora was avoided with the PSB. Among 10 baboons with bacteriologically documented pneumonias, the PSB cultures correctly identified the causative pathogen in 7 animals despite the presence of diffuse lung infiltrates radiographically, and multiple pathogenic bacteria in proximal airway secretions. Only 1 of 10 (10%) PSB specimens in these animals was contaminated with a possibly unrelated pathogen. The PSB largely avoided contamination of the culture specimen by proximal airway flora, and therefore should be useful in differentiating bacterial colonization of the airways from pneumonia in the presence of diffuse pulmonary infiltrates.