Golden Goal collaborates with Flamingo in conferring synaptic-layer specificity in the visual system
Golden Goal collaborates with Flamingo in conferring synaptic-layer specificity in the visual system
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DOI：
10.1038/nn.2756
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发表时间：
2011-03-01
影响因子：
25
通讯作者：
Suzuki, Takashi
中科院分区：
文献类型：
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作者：
Hakeda-Suzuki, Satoko;Berger-Mueller, Sandra;Suzuki, Takashi
神经元连接通常组织在包含具有相似功能的神经元之间的突触的层中。在果蝇中，R7和R8光感受器检测不同的波长，在不同的髓质层形成突触。突触层选择特异性的机制尚不清楚。我们发现，黄金目标（Gogo）和火烈鸟（FMI），两个细胞表面蛋白参与光感受器靶向，在R8感光轴突功能相互作用。我们的研究结果表明，Gogo促进R8感光轴突粘附到临时层M1，而Gogo和FMI合作，以介导轴突靶向最终层M3。结构-功能分析表明，Gogo和FMI通过Gogo胞质结构域与细胞内组分相互作用。此外，在靶细胞中，R8感光轴突靶向也需要Fmi。我们建议，Gogo作为一个功能合作伙伴的R8感光轴突靶向和动态调节它们的相互作用指定突触层选择的光感受器的FMI。
Neuronal connections are often organized in layers that contain synapses between neurons that have similar functions. In Drosophila, R7 and R8 photoreceptors, which detect different wavelengths, form synapses in distinct medulla layers. The mechanisms underlying the specificity of synaptic-layer selection remain unclear. We found that Golden Goal (Gogo) and Flamingo (Fmi), two cell-surface proteins involved in photoreceptor targeting, functionally interact in R8 photoreceptor axons. Our results indicate that Gogo promotes R8 photoreceptor axon adhesion to the temporary layer M1, whereas Gogo and Fmi collaborate to mediate axon targeting to the final layer M3. Structure-function analysis suggested that Gogo and Fmi interact with intracellular components through the Gogo cytoplasmic domain. Moreover, Fmi was also required in target cells for R8 photoreceptor axon targeting. We propose that Gogo acts as a functional partner of Fmi for R8 photoreceptor axon targeting and that the dynamic regulation of their interaction specifies synaptic-layer selection of photoreceptors.