Prostaglandin E1 analog increases spinal cord blood flow at the point of compression during and after experimental spinal cord injury
Prostaglandin E1 analog increases spinal cord blood flow at the point of compression during and after experimental spinal cord injury
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DOI：
10.1038/sc.2009.99
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发表时间：
2010-02-01
期刊：
影响因子：
2.2
通讯作者：
Yamamoto, H.
中科院分区：
文献类型：
--
作者：
Hamamoto, Y.;Ogata, T.;Yamamoto, H.
研究设计：采用大鼠脊髓受压模型进行活体实验。目的：评价前列腺素E1(PGE1)对大鼠胸段脊髓血流量的影响及对后肢运动功能的影响。背景：到目前为止，PGE1对受压点脊髓血流量的影响尚未被检测。暴露大鼠胸段脊髓，测量压迫前、压迫中、压迫后脊髓血流量。结果：在压迫期间，PGE1治疗组大鼠脊髓血流量明显高于未接受PGE1治疗的对照组大鼠。减压后，对照组大鼠脊髓血流量迅速恢复到受压前的60%左右。当动物接受PGE1治疗时，减压后的血流量达到压缩前水平的90%左右。20g压迫40min可致大鼠后肢运动障碍。应用PGE1可明显改善大鼠脊髓损伤后的后肢运动功能。结论：应用PGE1可增加脊髓受压过程中和脊髓受压后的脊髓血流量，改善脊髓损伤后的运动功能。脊髓(2010年)48,149153；doi：10.1038/sc.2009.99；在线发布
Study design: An in vivo study using a spinal cord compression model in rats.Objectives: To evaluate the effect of prostaglandin E1 (PGE1) on the change in thoracic spinal cord blood flow and on hind-limb motor function.Background: Until now, effect of PGE1 on spinal cord blood flow at the point of compression has not been tested.Methods: Our newly developed blood flow measurement system was a combination of a noncontact-type Laser Doppler system and a spinal cord compression device. The rat thoracic spinal cord was exposed and spinal cord blood flow at the point of compression was measured before, during and after compression. The functioning of the animals' hind-limbs was evaluated by the BBB Scale and by measuring the frequency of voluntary standing.Results: During the compression period, spinal cord blood flow was significantly higher in the PGE1-treated rats than in the control rats, which did not receive PGE1. After decompression, the spinal cord blood flow rapidly recovered to about 60% of the precompression level in the control rats. When the animals were treated with PGE1, blood flow after decompression reached about 90% of the precompression level. Twenty-gram compression for 40 mins induced motor deficiencies in the rat hind-limbs. The application of PGE1 significantly improved motor function of the rat hind-limbs after spinal cord injury.Conclusions: The application of PGE1 increased spinal cord blood flow during and after spinal cord compression, and improved motor function after the spinal cord injury. Spinal Cord (2010) 48, 149-153; doi: 10.1038/sc.2009.99; published online 18 August 2009