Association between plasma level of growth hormone and sex differentiation of hepatic steroid metabolism in the rat.
Association between plasma level of growth hormone and sex differentiation of hepatic steroid metabolism in the rat.
复制标题
大鼠血浆生长激素水平与肝脏类固醇代谢性别分化的关系。
作者：
A. Mode;J. Gustafsson;J. Jansson;S. Edén;O. Isaksson
在两种不同的动物模型中研究了生长激素 (GH) 的血浆模式调节大鼠肝脏类固醇代谢的假设：1) 通过以不同频率施用人 GH 或通过 Alzet 渗透微型泵向垂体切除的雌性大鼠连续输注激素来实现不同的 GH 血浆模式；2) 具有完整的垂体切除术的动物中 GH 的血浆模式 在导致肝脏类固醇代谢女性化的条件下对垂体进行了研究。通过研究不同时间从尾尖采集的血液样本中的最低和最高血浆 GH 水平来确定 GH 模式。对垂体切除的雌性大鼠连续给予人 GH 后，4-[4-14C]雄烯-3,17-二酮的肝微粒体代谢呈女性化，例如5α-还原酶和16α-羟化酶活性之间的比率（5α/16α比率）从2.45±0.21增加到31.2±1.76。当动物被...观察到类似的结果。
The hypothesis that the plasma pattern of growth hormone (GH) regulates hepatic steroid metabolism in the rat was studied in two different animal models: 1) different plasma patterns of GH were achieved by the administration of human GH at different frequencies or by infusing the hormone continuously by means of Alzet osmotic minipumps to hypophysectomized female rats and 2) the plasma pattern of GH in animals with an intact pituitary gland was investigated under conditions which lead to feminization of hepatic steroid metabolism. The pattern of GH was determined by studying minimum and maximum plasma GH levels in blood samples taken from the tip of the tail at various times. After continuous administration of human GH to hypophysectomized female rats, the liver microsomal metabolism of 4-[4-14C]androstene-3,17-dione was feminized, e.g. the ratio between 5α-reductase and 16α-hydroxylase activities (5α/16α ratio) increased from 2.45 ± 0.21 to 31.2 ± 1.76. Similar results were observed when the animals were...