Relaxing effect of interleukin‐1 on rat cultured Ito cells
Relaxing effect of interleukin‐1 on rat cultured Ito cells
复制标题
IL-1对大鼠培养的Ito细胞的松弛作用
作者：
Michiie Sakamoto;T. Ueno;H. Sugawara;T. Torimura;R. Tsuji;K. Sujaku;M. Sata;Ken Tanikawa
白细胞介素1受体(IL-1β，IL-1β)与肝脏疾病密切相关。IL-1β通过环鸟苷3‘，5’-单磷酸(CGMP)在血管平滑肌细胞中产生一氧化氮(NO)，起到松弛血管的作用。在本研究中，我们评价了IL-1β对培养的伊藤细胞的松弛作用。从雄性Wistar大鼠肝脏分离Ito细胞，培养24小时。激光共聚焦显微镜观察诱导型一氧化氮合酶(INOS)和cGMP的免疫定位及cGMP的荧光强度。用0、200、1000pmol/L IL-1β处理ITO细胞，12h后测定细胞内cGMP浓度。观察IL-1β作用于Ito细胞12h以上和未经IL-1β处理的细胞，比较加入IL-1β前后同一细胞的面积。接下来，观察了L精氨酸和S亚硝基-N-乙酰青霉胺对IL-1β诱导的伊藤细胞松弛的影响。在ITO细胞中，加入IL-1β后，可观察到诱导型一氧化氮合酶的免疫荧光，且cGMP的荧光强度增强。加入IL-1β后，细胞内cGMP浓度呈剂量依赖性增加。IL-1β处理组细胞面积较未处理组显著增加。L可剂量依赖地抑制IL-1β对ITO细胞的松弛作用，但可增强SNAP的松弛作用。这些结果表明，IL-1β在体外培养的ITO细胞中产生NO，并通过cGMP起松弛作用。
Interleukin‐1β (IL‐1β) is closely involved in liver disorders. IL‐1β produces nitric oxide (NO) in vascular smooth muscle cells and relaxes vascular smooth muscle via cyclic guanosine 3′,5′‐monophosphate (cGMP). In this study, we evaluated the relaxing effect of IL‐1β on cultured Ito cells. Ito cells were isolated from the livers of male Wistar rats and cultured for 24 hours. Immunolocalization of inducible nitric oxide synthase (iNOS) and cGMP and intensity of fluorescence of cGMP were examined using a confocal laser microscope. Ito cells were treated with 0, 200, and 1,000 pmol/L IL‐1β, and the intracellular cGMP concentration was measured after 12 hours. Moreover, Ito cells treated with 200 and 1,000 pmol/L IL‐1β and not treated with IL‐1β were observed over 12 hours, and the area of the same Ito cell was compared before and after the addition of IL‐1β. Next, effects of NG‐monomethyl‐L‐arginine (L‐NMMA) and S‐nitroso‐N‐acetyl‐DL‐penicillamine (SNAP) on Ito cell relaxation by IL‐1β treatment were examined. In Ito cells, immunofluorescence of iNOS was observed, and fluorescent intensity of cGMP increased after addition of IL‐1β. Intracellular cGMP concentration increased dose‐dependently after addition of IL‐1β. Cell area significantly increased in the IL‐1β‐treated group compared with the untreated group. Relaxation of Ito cells by IL‐1β treatment was inhibited by L‐NMMA in a dose‐dependent manner, but was enhanced by SNAP. These results indicate that IL‐1β produces NO in cultured Ito cells and relaxes the cells via cGMP.
DOI：
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发表时间：
1981-09
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
L. Ignarro;H. Lippton;J. Edwards;W. Baricos;A. Hyman;P. Kadowitz;C. A. Gruetter
通讯作者：
L. Ignarro;H. Lippton;J. Edwards;W. Baricos;A. Hyman;P. Kadowitz;C. A. Gruetter
影响因子：
56.9
作者：
XIE, QW;CHO, HJ;NATHAN, C
通讯作者：
NATHAN, C