Evidence for Differentiation of Colon Tissue Microbiota in Patients with and without Postoperative Hirschsprung's Associated Enterocolitis: A Pilot Study.
Evidence for Differentiation of Colon Tissue Microbiota in Patients with and without Postoperative Hirschsprung's Associated Enterocolitis: A Pilot Study.
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DOI：
10.5223/pghn.2021.24.1.30
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Alekseyenko AV
中科院分区：
文献类型：
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作者：
Arbizu RA;Collins D;Wilson RC;Alekseyenko AV
目的探讨先天性巨结肠症(HSCR)患者术后合并先天性巨结肠(HAEC)病史与无HAEC病史患者结肠微生物区系的差异。用细菌脱氧核糖核酸(DNA)提取和16S rDNA测序鉴定结肠组织微生物区系，进行分类比较。在有HAEC术后病史的患者样本中，样本中的序列多样性丰富度显著较高。我们观察到HAEC患者中类杆菌、纤毛虫和蓝藻的相对丰度增加，HSCR患者中梭菌、放线菌和变形杆菌的相对丰富，HAEC患者中Dolosigranulum、Roseouria和链球菌的相对丰富，HSCR患者中丙酸杆菌和Delftia的相对丰富。我们的发现提供了证据，证明HSCR患者术后HAEC的结肠组织微生物区系组成是不同的。
To investigate the differences in the colon microbiota composition of Hirschsprung's disease (HSCR) patients with and without a history of postoperative Hirschsprung's associated enterocolitis (HAEC). Colon tissue microbiota was characterized by bacterial deoxyribonucleic acid (DNA) extraction and 16S rDNA sequencing for taxonomic classification and comparison. The sequence diversity richness within samples was significantly higher in samples from patients with a history of postoperative HAEC. We observed an increased relative abundance of the phyla Bacteroidetes, Firmicutes and Cyanobacteria in HAEC patients and Fusobacteria, Actinobacteria and Proteobacteria in HSCR patients and, an increased relative abundance of the genera Dolosigranulum, Roseouria and Streptococcus in HAEC patients and Propionibacterium and Delftia in HSCR patients. Our findings provide evidence that the colon tissue microbiota composition is different in HSCR patients with and without postoperative HAEC.