Molecular characterization of the Japanese encephalitis virus representative immunotype strain JaGAr 01
Molecular characterization of the Japanese encephalitis virus representative immunotype strain JaGAr 01
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DOI：
10.1016/s0168-1702(98)00130-0
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发表时间：
1999-01-01
期刊：
影响因子：
5
通讯作者：
Takegami, T
中科院分区：
文献类型：
--
作者：
Mangada, MNM;Takegami, T
我们测定了日本脑炎病毒JaGAr 01株的全基因组序列及其预测的氨基酸序列。与10株全序列的比较表明，核苷酸同源性为89.62%至99.49%。氨基酸序列同源性范围为96.85%至99.74%。氨基酸序列的比较显示，JaGAr 01在E蛋白的位置123处具有独特的氨基酸精氨酸，而其他八种菌株含有丝氨酸。通过自由能最小化的二级结构预测显示了JaGAr 01的独特结构，其包括所有黄病毒保守的RNA片段。推测这些结果可能在JaGAr 01的复制和抗原特性中发挥的作用。系统发育分析表明，JAE原型株中山株、JAE 01株和北京1号株的E蛋白在氨基酸特征上存在差异。通过对核酸和蛋白质序列的邻位连接和Fitch-Margoliash分析计算的系统发育树显示中山和北京在一个不同于JaGAr 01的簇中。(C)1999年由Elsevier Science B. V.出版，版权所有。
We determined the full genomic sequence of the Japanese encephalitis virus JaGAr 01 strain and its predicted amino acid sequence. Nucleotide sequence comparison with ten fully sequenced JE strains shows a homology range from 89.62 to 99.49%. Amino acid sequence homologies range from 96.85 to 99.74%. Comparison of amino acid sequences shows a unique amino acid, arginine, for JaGAr 01 at position 123 of the E-protein, while the eight other strains contained serine. Secondary structure prediction by free energy minimization shows a unique structure for JaGAr 01 that includes an RNA segment that is conserved for all flaviviruses. Speculation is made about the role these results may play in the replication and antigenic characteristics of JaGAr 01. Phylogenetic analyses of the E-protein of JaGAr 01 together with 35 other JE strains showed diversity in amino acid characteristics between the prototype strains Nakayama, JaGAr 01 and Beijing-l. Phylogenetic trees computed by neighbor joining and Fitch-Margoliash analysis of nucleic acid and protein sequences showed Nakayama and Beijing in one cluster different from JaGAr 01. (C) 1999 Published by Elsevier Science B.V. All rights reserved.