MOLECULAR-CLONING AND SEQUENCE COMPARISON OF THE S1 GLYCOPROTEIN OF THE GRAY AND JMK STRAINS OF AVIAN INFECTIOUS-BRONCHITIS VIRUS
MOLECULAR-CLONING AND SEQUENCE COMPARISON OF THE S1 GLYCOPROTEIN OF THE GRAY AND JMK STRAINS OF AVIAN INFECTIOUS-BRONCHITIS VIRUS
复制标题
DOI：
10.1007/bf01702878
复制
发表时间：
1995-02-01
期刊：
影响因子：
1.6
通讯作者：
JACKWOOD, MW
中科院分区：
文献类型：
--
作者：
KWON, HM;JACKWOOD, MW
测定了禽传染性支气管炎病毒（IBV） Gray株和JMK株S1糖蛋白基因的核苷酸序列，并与已发表的IBV序列进行了比较。灰色IBV与JMK株核苷酸序列相似性达99%。与其他IBV毒株相比，Gray和JMK毒株的总核苷酸序列相似性在82.0% ~ 87.4%之间，预测的S1糖蛋白氨基酸序列相似性为98.8%，在10个氨基酸序列差异中，有6个位于残基99 ~ 127之间，表明该区域可能在病毒的组织营养作用中起作用。Gray和JMK菌株S1糖蛋白与其他IBV菌株序列氨基酸相似性为79.5% ~ 84.6%，其中Gray和JMK菌株S1和S2糖蛋白亚基之间的蛋白酶裂解位点为丝氨酸而非苯丙氨酸，与Ark99和SE17菌株序列相似。这种氨基酸变化的意义尚不清楚。根据Gray和JMK菌株的核苷酸序列，通过计算机分析选择BsmAI限制性内切酶，进行限制性内切片段长度多态性分析，区分两株菌株。
The nucleotide sequences of S1 glycoprotein genes of the Gray and JMK strains of avian infectious bronchitis virus (IBV) were determined and compared with published sequences for IBV. The IBV Gray and JMK strains had 99% nucleotide sequence similarity. The overall nucleotide sequence similarity of the Gray and JMK strains compared with other IBV strains was between 82.0% and 87.4%, The similarity of the predicted amino acid sequence for the S1 glycoproteins of the Gray and JMK strains was 98.8%, Six of the 10 differences in the amino acid sequence were found between residues 99 and 127, suggesting a possible role for that region in the tissue trophisms of the viruses, The S1 glycoprotein of the Gray and JMK strains had 79.5%-84.6% amino acid similarity with the published sequence of other IBV strains, Serine instead of phenylalanine was observed in the protease cleavage site between the S1 and S2 glycoprotein subunits for the Gray and JMK strains, which was similar to the published sequence for the Ark99 and SE17 strains. The significance of that amino acid change is not known. Based on the nucleotide sequence of the Gray and JMK strains, the BsmAI restriction enzyme was selected by computer analysis and was used in restriction fragment length polymorphism analysis to differentiate the two strains.