Melatonin prevents oxidative stress and changes in antioxidant enzyme expression and activity in the liver of aging rats (Retracted Article)
Melatonin prevents oxidative stress and changes in antioxidant enzyme expression and activity in the liver of aging rats (Retracted Article)
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DOI：
10.1111/j.1600-079x.2006.00409.x
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发表时间：
2007-04-01
影响因子：
10.3
通讯作者：
Gonzalez-Gallego, Javier
中科院分区：
文献类型：
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作者：
Mauriz, Jose L.;Molpeceres, Virginia;Gonzalez-Gallego, Javier
本研究比较了补充褪黑激素对年轻（3 个月大）和老年（24 个月大）大鼠肝脏中氧化应激标志物以及抗氧化酶活性和表达的影响。动物在饮用水中补充褪黑激素（20 mg/L）4周。硫代巴比妥反应物质（TBARS）的肝脏浓度（作为脂质过氧化的指标）和氧化型谷胱甘肽与还原型谷胱甘肽的比率在老年大鼠中显着增加（+ 58％），而接受褪黑激素的老年动物的值与年轻大鼠没有显着差异。老年大鼠的肝脏中铜、锌超氧化物歧化酶（SOD）（-25%）、胞质（-21%）和线粒体（-40%）谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）（-34%）的活性显着降低。褪黑素消除了这些变化，并且还阻止了 Cu,Zn-SOD (-33%)、胞浆 GPx (-30%) 和线粒体 GPx (-47%) 肝脏蛋白含量的减少（通过蛋白质印迹法测量）。褪黑激素还消除了衰老引起的 Cu、Zn-SOD mRNA (-39%) 和 GPx mRNA (-86%) 水平的降低。总之，我们的数据表明，褪黑素治疗消除了老年大鼠肝脏中的氧化应激，并且防止 CAT 活性降低以及 Cu、Zn-SOD 和 GPx 基因表达的下调有助于这种效果。
This study compared the effects of melatonin supplementation on markers of oxidative stress, and on the activity and expression of antioxidant enzymes in the liver of young (3-month-old) and aging (24-month-old) rats. Animals were supplemented with melatonin in the drinking water (20 mg/L) for 4 wk. Liver concentration of thiobarbituric-reactive substances (TBARS), as an index of lipid peroxidation, and the oxidized to reduced glutathione ratio significantly increased in aged rats (+ 58%), while values did not significantly differ from the young in aged animals receiving melatonin. Significant decreases in the liver activities of Cu,Zn-superoxide dismutase (SOD) (-25%), cytosolic (-21%) and mitochondrial (-40%) glutathione peroxidase (GPx), and catalase (CAT) (-34%) were found in aged rats. Melatonin abolished these changes and also prevented the reduction of Cu,Zn-SOD (-33%), cytosolic GPx (-30%), and mitochondrial GPx (-47%) liver protein content as measured by Western blot. Reductions in Cu,Zn-SOD mRNA (-39%), and GPx mRNA (-86%) levels induced by aging were also abolished by melatonin. In summary, our data indicate that melatonin treatment abrogates oxidative stress in the liver of aged rats, and that prevention of the decreased activity of CAT and the downregulation of Cu,Zn-SOD and GPx gene expression contribute to this effect.