KNAT6:: an Arabidopsis homeobox gene involved in meristem activity and organ separation
KNAT6:: an Arabidopsis homeobox gene involved in meristem activity and organ separation
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DOI：
10.1105/tpc.106.041988
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发表时间：
2006-08-01
期刊：
影响因子：
11.6
通讯作者：
Pautot, Veronique
中科院分区：
文献类型：
--
作者：
Belles-Boix, Enric;Hamant, Olivier;Pautot, Veronique
同源异型盒基因家族在多细胞生物的发育过程中起着至关重要的作用。来自拟南芥的KNOTTED样基因（KNAT 6和KNAT 2）是分生组织基因SHOOT MERISTEMLESS（STM）和BREVIPEDICELLUS的近亲，但它们的功能目前尚不清楚。为了研究它们的作用，我们鉴定了KNAT 6和KNAT 2的无效等位基因。我们证明，KNAT 6有助于冗余STM的茎尖分生组织（SAM）和器官分离的维护。与此作用一致，SAM中KNAT 6的表达结构域标记SAM和子叶之间的边界。knat 6 stm-2双突变体分生活性的缺乏和子叶的融合与杯形子叶（CUC）活性的调节有关。在胚胎发生过程中，KNAT 6的表达晚于STM和CUC。与此事实一致，CUC 1和CUC 2是KNAT 6表达所必需的。这些数据提供了一个模型的基础，其中KNAT 6有助于SAM的维护和边界建立在胚胎中通过STM/CUC途径。KNAT 2虽然是KNAT 6家族中最接近的成员，但却没有这样的功能。
The homeobox gene family plays a crucial role during the development of multicellular organisms. The KNOTTED-like genes from Arabidopsis thaliana (KNAT6 and KNAT2) are close relatives of the meristematic genes SHOOT MERISTEMLESS (STM) and BREVIPEDICELLUS, but their function is not currently known. To investigate their role, we identified null alleles of KNAT6 and KNAT2. We demonstrate that KNAT6 contributes redundantly with STM to the maintenance of the shoot apical meristem (SAM) and organ separation. Consistent with this role, the expression domain of KNAT6 in the SAM marks the boundaries between the SAM and cotyledons. The lack of meristematic activity in the knat6 stm-2 double mutant and the fusion of cotyledons were linked to the modulation of CUP-SHAPED COTYLEDON (CUC) activity. During embryogenesis, KNAT6 is expressed later than STM and CUC. In agreement with this fact, CUC1 and CUC2 were redundantly required for KNAT6 expression. These data provide the basis for a model in which KNAT6 contributes to SAM maintenance and boundary establishment in the embryo via the STM/CUC pathway. KNAT2, although the closest related member of the family to KNAT6, did not have such a function.