RNA-Seq analysis of rye-grass transcriptomic response to an herbicide inhibiting acetolactate-synthase identifies transcripts linked to non-target-site-based resistance
RNA-Seq analysis of rye-grass transcriptomic response to an herbicide inhibiting acetolactate-synthase identifies transcripts linked to non-target-site-based resistance
复制标题
DOI：
10.1007/s11103-015-0292-3
复制
发表时间：
2015-03-01
影响因子：
5.1
通讯作者：
Delye, Christophe
中科院分区：
文献类型：
--
作者：
Duhoux, Arnaud;Carrere, Sebastien;Delye, Christophe
除草剂的非靶点抗性（NTSR）破坏了农业杂草控制，是一个全球性的粮食安全问题。NTSR被认为是抗性植物中由差异基因调控驱动的多基因适应性性状。关于它的遗传决定论知之甚少，这排除了NTSR诊断和进化研究。我们使用Illumina RNA测序来研究全球主要杂草黑麦草中对乙酰乳酸合酶（ALS）抑制性除草剂啶磺草胺敏感或耐药的植物之间的转录组差异。在施用除草剂之前和施用除草剂之后沿着时间进程收集植物。从头转录组组装产生了包括92，381个重叠群的资源（LOLbase），所述重叠群代表被分配推定注释的潜在活性转录物。在抗性和敏感植物中观察到与文献一致的ALS抑制的早期效应，证明LOLbase数据与研究除草剂响应相关。抗性和敏感植物的比较鉴定了30个候选NTSR重叠群。使用212株对啶磺草胺和/或对ALS抑制剂碘磺隆+甲基磺隆具有抗性或敏感性的植物进行进一步验证，证实了四个重叠群（两个细胞色素P450，一个糖基转移酶和一个谷胱甘肽-S-转移酶）是NTSR标记，其组合表达水平可以可靠地鉴定抗性植物。这项工作证实了NTSR是由差异基因表达驱动的，涉及不同的机制。它为后续的NTSR调查提供了工具和基础。
Non-target-site resistance (NTSR) to herbicides that disrupts agricultural weed control is a worldwide concern for food security. NTSR is considered a polygenic adaptive trait driven by differential gene regulation in resistant plants. Little is known about its genetic determinism, which precludes NTSR diagnosis and evolutionary studies. We used Illumina RNA-sequencing to investigate transcriptomic differences between plants from the global major weed rye-grass sensitive or resistant to the acetolactate-synthase (ALS) inhibiting herbicide pyroxsulam. Plants were collected before and along a time-course after herbicide application. De novo transcriptome assembly yielded a resource (LOLbase) including 92,381 contigs representing potentially active transcripts that were assigned putative annotations. Early effects of ALS inhibition consistent with the literature were observed in resistant and sensitive plants, proving LOLbase data were relevant to study herbicide response. Comparison of resistant and sensitive plants identified 30 candidate NTSR contigs. Further validation using 212 plants resistant or sensitive to pyroxsulam and/or to the ALS inhibitors iodosulfuron + mesosulfuron confirmed four contigs (two cytochromes P450, one glycosyl-transferase and one glutathione-S-transferase) were NTSR markers which combined expression levels could reliably identify resistant plants. This work confirmed that NTSR is driven by differential gene expression and involves different mechanisms. It provided tools and foundation for subsequent NTSR investigations.