WBC27, an Adenosine Tri-phosphate-binding Cassette Protein, Controls Pollen Wall Formation and Patterning in Arabidopsis
WBC27, an Adenosine Tri-phosphate-binding Cassette Protein, Controls Pollen Wall Formation and Patterning in Arabidopsis
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WBC27 是一种三磷酸腺苷结合盒蛋白，控制拟南芥花粉壁的形成和图案形成
DOI：
10.1111/j.1744-7909.2010.01010.x
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发表时间：
2011-01-01
影响因子：
11.4
通讯作者：
Ye, De
中科院分区：
文献类型：
--
作者：
Dou, Xiao-Ying;Yang, Ke-Zhen;Ye, De
在开花植物中，外壁成分源自绒毡层。尽管外壁物质对有性植物繁殖很重要，但人们对外壁物质从绒毡层到发育中的小孢子的易位知之甚少。在这里，我们报告了拟南芥 WBC27 基因的功能特征。 WBC27 编码腺苷三磷酸结合盒 (ABC) 转运蛋白，并优先在绒毡层中表达。 WBC27 的突变破坏了外壁的形成。 wbc27突变体小孢子从四分体释放后开始退化，大部分小孢子在单核阶段崩溃。只有少量的wbc27-1小孢子能够发育成三细胞花粉粒。这些存活的花粉粒缺乏外壁，在开花前在花药中萌发。所有这些结果表明，ABC 转运蛋白 WBC27 在拟南芥外壁的形成中发挥着重要作用，可能是通过将孢粉质的脂质前体从绒毡层易位到发育中的小孢子来实现的。
In flowering plants, the exine components are derived from tapetum. Despite its importance to sexual plant reproduction, little is known about the translocation of exine materials from tapetum to developing microspores. Here we report functional characterization of the arabidopsis WBC27 gene. WBC27 encodes an adenosine tri-phosphate binding cassette (ABC) transporter and is expressed preferentially in tapetum. Mutation of WBC27 disrupted the exine formation. The wbc27 mutant microspores began to degenerate once released from tetrads and most of the microspores collapsed at the uninucleate stage. Only a small number of wbc27-1 microspores could develop into tricellular pollen grains. These survival pollen grains lacked exine and germinated in the anther before anthesis. All of these results suggest that the ABC transporter, WBC27 plays important roles in the formation of arabidopsis exine, possibly by translocation of lipidic precursors of sporopollenin from tapetum to developing microspores.